Because of the high unsaturated fatty acid content of dried distillers grains with solubles (DDGS), usage of DDGS as a protein supplement for dairy cows is limited. However, new extraction processes can remove corn oil from distillers grains and produce solvent extracted distillers grain with solubles (NM; NovaMeal, Brookings, SD). The objective of this study was to evaluate the impact of replacing canola meal with NM and urea as protein sources on milk yield and components in a commercial dairy herd. A total of 2,776 Holstein cows averaging 63 DIM were enrolled to one of 4 dietary treatment pens: 1) primiparous control (C) group with 3.1 kg CM, 0.058 kg urea; 2) primiparous treatment (NT) group replaced CM with 2.1 kg NM, 0.11 kg urea; 3) multiparous C group 3.5 kg CM, 0.057 kg urea; and 4) multiparous NT group replaced CM with 2.4 kg NM, 0.11 kg urea. The TMR were formulated to be isonitrogenous and isoenergetic with similar fatty acid profiles and averaged 17% CP, 4.5% fat and contained 45% forage and 55% concentrate. Pens were fed for 6 wk, then TMR for C and NT pens were switched within parity groups for a total of 2 pens per treatment-parity with 694 cows per treatment-parity group. Then, following a 2 wk adjustment period, data collection resumed. Data were analyzed using the Mixed procedure of SAS (Statistical Analysis System, v.9.4) with random effects DIM and days on treatment. Multiparous cows had greater milk yield, ECM, milk fat yield, milk protein yield, and DMI but, lower milk fat %, milk protein % and MUN than primiparous cows (P < 0.05) due to their higher milk yield. There were no differences between treatment least squares means (LSM) for milk yield, ECM, milk protein %, DMI and MUN but, milk fat yield and % were greater with treatment NT (2.05 kg vs. 2.01 kg and 5.29% vs. 5.07% LSM for NT and C treatments, respectively). Milk protein yield was lower in treatment NT (1.25 kg vs. 1.28 kg LSM for NT and C treatments, respectively). These results show that NM with supplemented urea can be used to replace CM as a protein supplement in a lactating dairy cattle TMR.