Abstract #131

# 131
Reduction of aflatoxin M1 in milk of Holstein cows administered an aluminosilicate clay.
S. C. Allen*1, K. N. Russo2, D. M. Paulus Compart2, S. H. Ward1, 1North Carolina State University, Raleigh, NC, 2PMI Nutrition Additives, Arden Hills, MN.

Thirty-five Holstein cows were utilized in a randomized complete block design to evaluate the efficacy of an aluminosilicate clay (adsorbent, FloMatrix, PMI Nutritional Additives, Arden Hills, MN) at reducing aflatoxin M1 transfer into the milk. Cows were stratified by parity, stage of lactation, and previous milk production. Cows were randomly assigned to 1 of 5 dietary treatments, and treatment was administered for 13 d (n = 7): (1) control (CON), basal TMR with no AF or clay; (2) clay control (4C), basal TMR plus 4 oz clay; (3) AF control (AFC), basal TMR plus 113 ppb AF; (4) AF diet with smaller clay dose (4C+AF), basal TMR plus 4 oz clay and 113 ppb AF; (5) AF diet with greater clay dose (8C+AF) basal TMR plus 8 oz clay and 113 ppb AF. Data were analyzed using the GLM procedure of SAS. Treatment and day were considered independent variables, and milk yield, DM and nutrient intakes, aflatoxin M1 variables, milk composition, body weight and condition, locomotion, and respiratory rate were dependent variables. A covariate adjustment was used to analyze milk yield and composition from previous milk yield records and DHIA records taken 3 d before the start of the treatment period. Means were separated using Fisher’s Least Significant Difference, and significance was declared when P ≤ 0.05. Tendencies were discussed when 0.05 < P ≤ 0.10. Data were presented as mean ± the largest standard error. Milk yield averaged 29.63, 31.85, 29.95, 29.93, and 31.30 ± 1.762 kg/d for CON, 4C, AFC, 4C+AF, and 8C+AF diets, respectively, and was greatest in cows consuming 4C and 8C+AF diets (P = 0.013). Respiratory rate and BCS were similar across treatments, and 8C+AF cows tended to have a greater locomotion score compared with other treatments (1.71 v. 1.23, P = 0.057). Aflatoxin M1 concentration, secretion, and transfer were reduced by the addition of clay (P < 0.001), and a dose response was observed. Aflatoxin M1 concentration averaged < 0.01, 0.00, 1.64, 1.26, and 0.90 ± 0.383 ppb for CON, 4C, AFC, 4C+AF, and 8C+AF cows, respectively. A reduction of transfer of 21.88 and 40.63% was observed for 4C+AF and 8C+AF diets, respectively.

Key Words: clay adsorbent, aflatoxin, milk production

Speaker Bio
Sarah Allen is a doctoral student at North Carolina State University. Sarah is originally from Little Rock, Arkansas, and received her bachelor’s and master’s degrees from Mississippi State University. Sarah’s research interests include aflatoxin mitigation in dairy cow diets and utilizing milk fatty acid composition to optimize production.