Abstract #T18
Section: Animal Health (posters)
Session: Animal Health Posters 2
Format: Poster
Day/Time: Tuesday 7:30 AM–9:30 AM
Location: Exhibit Hall A
Session: Animal Health Posters 2
Format: Poster
Day/Time: Tuesday 7:30 AM–9:30 AM
Location: Exhibit Hall A
# T18
Effects of vaccinating against brucellosis and clostridia simultaneously or not on the immune response of dairy calves.
H. C. Diniz Neto1, C. F. A. Lage*1, M. C. Lombardi1, W. A. Carvalho2, F. S. Machado2, L. G. R. Pereira2, T. R. Tomich2, M. M. Campos2, C. P. Ramos1, R. A. Assis1, R. O. S. Silva1, F. C. F. Lobato1, E. M. S. Dorneles3, A. P. Lage1, S. G. Coelho1, 1Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil, 2Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), Juiz de Fora, MG, Brazil, 3Universidade Federal de Lavras, Lavras, MG, Brazil.
Key Words: antibody, cellular proliferation, serum neutralization
Effects of vaccinating against brucellosis and clostridia simultaneously or not on the immune response of dairy calves.
H. C. Diniz Neto1, C. F. A. Lage*1, M. C. Lombardi1, W. A. Carvalho2, F. S. Machado2, L. G. R. Pereira2, T. R. Tomich2, M. M. Campos2, C. P. Ramos1, R. A. Assis1, R. O. S. Silva1, F. C. F. Lobato1, E. M. S. Dorneles3, A. P. Lage1, S. G. Coelho1, 1Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil, 2Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), Juiz de Fora, MG, Brazil, 3Universidade Federal de Lavras, Lavras, MG, Brazil.
The objective of this research was to evaluate the effects of vaccinating simultaneously or not against brucellosis and clostridia on the immune response. Fifty female dairy calves were enrolled (38 Gyr and 12 5/8 Holstein-Gyr). At 120 d of age animals were distributed into the 3 treatments: B (n = 18) vaccine only against brucellosis (B19- 2 mL subcutaneously); C (n = 14) vaccine only against Clostridium spp. (inactive culture of C. chauvoei and toxoids from C. botulinum tipo C and D, C. septicum, C. novyi, C. perfringens type B, C and D and C. sordellii, 5 mL subcutaneously) and CB (n = 18) a combination of both vaccine protocols (2 mL and 5 mL subcutaneously, respectively). Animals from groups C and CB were revaccinated against clostridia 28 d after the first vaccination. Blood sampling was performed on time 0 and on the 28th day for evaluation of the vaccine response against brucellosis; and on time 0 and 14 d after the booster to evaluate the immune response of the vaccine against selected clostridia agents. Serum neutralization was conducted for titration of antibodies anti-toxin epsilon from C. perfringens type D and botulinum from C. botulinum type C. For the analysis of the response against Brucella a cellular proliferation assay was performed. The software R was used for statistical analysis of the data. Values of P < 0.05 were considered significant. The medium of antibody titration against epsilon toxin was 14.57 UI/mL on group C, higher than for animals in group CB (9.20 UI/mL; P = 0,03). Similarly, animals from group CB also presented medium titration (1.44 UI/mL) against toxin C inferior to those observed in group C (4,92 UI/mL; P = 0,03). No differences were observed in the percentage of total lymphocytes and total proliferated; proliferated lymphocytes CD4+ and CD4+; proliferated lymphocytes CD8+ and CD8+ on group B and CB. These results suggest that the vaccine against brucellosis interferes on the humoral immune response of the tested clostridia antigens when the vaccines are simultaneously administrated.
Key Words: antibody, cellular proliferation, serum neutralization
