Abstract #T81

# T81
The effects of Lactobacillus diolivorans on fermentation characteristics and aerobic stability of corn silage.
K. Z. Moyer*3, E. A. Hellings3, D. M. Costa1,3, E. M. Santos2,3, N. A. Moyer3, L. Kung3, 1Universidade Federal da Lavras, Lavras, MG, Brazil, 2Universidade Federal da Paraíba, João Pessoa, Paraíba, Brazil, 3University of Delaware, Newark, DE.

The objective of this study was to evaluate the effectiveness of Lactobacillus diolivorans 1k202752 (LD, Provita Supplements Inc., Mendota Heights, MN) on the fermentation and aerobic stability of corn silage. Whole plant corn (38% DM) was untreated (UN) or treated with LD (theoretical application rate of 250,000 cfu/g of fresh forage). Four individually replicated silos were packed (208 kg of DM/m3) for each treatment into 7.5 L silos and opened after 3, 14, 30, 49, and 90 d of ensiling. Silos opened on d 14, 30, and 49 were subjected to air stress for 24 h: on d 7 for 14 d silos, on d 7 and 23 for 30 d silos, and on d 28 and d 42 for 49 d silos. Air-stressed silos had 3 1.60-cm holes plugged with stoppers and silicone glue, 2 located on the bottom of the bucket at opposing ends, and one on the lid of the bucket that were opened during times of air stress. 90 d silos had no air stress. Silages were analyzed for numbers of yeasts, fermentation end products, and aerobic stability (AS, h before a 2°C increase above baseline after exposure to air at 21°C). An additional set of silos at 90 d was subjected to aerobic stability measurements at 29°C. Data were analyzed using the Fit Model in JMP (SAS Institute Inc., Cary, NC) as a 2 × 5 factorial arrangement of treatments with the main effects of treatment, day, and their interaction. Silage with LD was lower (P < 0.001) in yeasts than UN after 30 (4.13 vs. 5.80 log cfu/g), 49 (3.60 vs. 6.21), and 90 d (3.35 vs. 4.33). Silage with LD had higher (P < 0.001) AS than UN at 49 (200 vs. 20 h) and 90 d (262 vs. 68 h). After 90 d of ensiling, UN differed (P < 0.001) from LD for lactic acid (4.95 vs. 2.83%), acetic acid (1.55 vs. 3.47%), 1,2 propanediol (0.10 vs. 0.27%), 1-propanol (0.00 vs. 0.52%), and propionic acid (0.17 vs. 0.54%). Exposure to the higher temperature for 90 d silage reduced (P < 0.005) AS for UN silage (68 h at 21°C vs. 37 h at 29°C) but not for LD (262 h for both temperatures). Addition of LD markedly improved the aerobic stability of corn silage even with air stress and at hot temperatures of spoilage.

Key Words: Lactobacillus diolivorans, aerobic stability, silage