Abstract #T127
Section: Ruminant Nutrition (posters)
Session: Ruminant Nutrition: Carbohydrates
Format: Poster
Day/Time: Tuesday 7:30 AM–9:30 AM
Location: Exhibit Hall A
Session: Ruminant Nutrition: Carbohydrates
Format: Poster
Day/Time: Tuesday 7:30 AM–9:30 AM
Location: Exhibit Hall A
# T127
Effects of aflatoxin with or without binders on in vitro rumen fermentation dynamics.
Y. Jiang*1, I. M. Ogunade2, K. G. Arriola1, A. A. Pech-Cervantes1, D. H. Kim1, Y. L. Xue3, X. Li4, D. Vyas1, A. T. Adesogan1, 1Department of Animal Sciences, University of Florida, Gainesville, FL, 2Division of Food and Animal Science, Kentucky State University, Frankfort, KY, 3Inner Mongolia Academy of Agriculture and Animal Husbandry Science, Hohhot, Inner Mongolia, China, 4School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
Key Words: rumen fermentation, aflatoxin, binder
Effects of aflatoxin with or without binders on in vitro rumen fermentation dynamics.
Y. Jiang*1, I. M. Ogunade2, K. G. Arriola1, A. A. Pech-Cervantes1, D. H. Kim1, Y. L. Xue3, X. Li4, D. Vyas1, A. T. Adesogan1, 1Department of Animal Sciences, University of Florida, Gainesville, FL, 2Division of Food and Animal Science, Kentucky State University, Frankfort, KY, 3Inner Mongolia Academy of Agriculture and Animal Husbandry Science, Hohhot, Inner Mongolia, China, 4School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
The effects of aflatoxin B1 (AFB1), with or without bentonite clay (CL) and Saccharomyces cerevisiae fermentation production (SCFP)-based aflatoxin binders on in vitro rumen fermentation of a dairy cow TMR was examined. The corn silage-based TMR (0.5 g, 17.3% CP and 1.67 Mcal/kg NEL) was incubated in duplicate in a rumen fluid – buffer inoculum (1:2 ratio; 50 mL) containing the following treatments: 1) Control (nothing); 2) T (37.5 ng of AFB1); 3) CL (T + 40 mg of CL); 4) CL+SCFP (CL + 7 mg of SCFP). The ruminal fluid was collected from 3 cannulated cows fed the same TMR 3 h after the morning feeding. Each treatment was incubated at 39°C for 4, 8, 16 and 24 h in each of 3 runs. Data were analyzed using Glimmix procedure of SAS. The model included fixed effect of treatment, random effects of cow, run and cow × run. Adding T reduced (P < 0.05) total volatile fatty acid (VFA) concentration after 4 and 8 h and molar proportion of propionic acid after 4 and 24 h of incubation, relative to control. Adding binders with T did not affect total VFA concentration after 4 and 8 h, but increased (P < 0.05) the measure after 16 h and tended to increase (P = 0.10) molar proportion of propionic acid after 24 h, compared with T. At 24 h, T had lower (P = 0.04) DMD and higher NH3-N (P = 0.04) concentration compared with the control as well as lower (P < 0.05) pH compared with the binders. Thus, AFB1, even at very low concentration (0.75 µg/L), had detrimental effects on the fermentation and DMD of the TMR. Adding binders to T did not prevent the negative effects of T within 8 h of incubation did so after 16 h or 24 h of incubation.
Table 1 (Abstr. T127). Effects of AFB1 with or without binders on in vitro rumen fermentation dynamics
| Treatment | SEM | Contrast P-value | ||||||
| Con | T | CL | CL+SCFP | Con vs. T | T vs. | CL vs. CL+SCFP | ||
| Total VFA, mM | ||||||||
| 4h | 110 | 91.1 | 93.8 | 101 | 12.8 | 0.02 | 0.38 | 0.39 |
| 8h | 128 | 104 | 106 | 98.5 | 9.21 | 0.01 | 0.80 | 0.35 |
| 16h | 105 | 99.1 | 127 | 114 | 10.4 | 0.54 | 0.01 | 0.22 |
| 24 h | 132 | 135 | 135 | 151 | 17.1 | 0.82 | 0.45 | 0.21 |
| Fermentation parameters, 24 h | ||||||||
| pH | 6.64 | 6.61 | 6.67 | 6.68 | 0.05 | 0.24 | 0.01 | 0.75 |
| NH3-N, mM/dL | 11.8 | 12.4 | 11.8 | 12.4 | 0.80 | 0.01 | 0.15 | 0.03 |
| DMD, % | 57.2 | 53.2 | 52.7 | 51.6 | 2.96 | 0.04 | 0.52 | 0.57 |
Key Words: rumen fermentation, aflatoxin, binder
