Abstract #W160
Section: Ruminant Nutrition (posters)
Session: Ruminant Nutrition: Ruminal Fermentation and Gas Production
Format: Poster
Day/Time: Wednesday 7:30 AM–9:30 AM
Location: Exhibit Hall A
Session: Ruminant Nutrition: Ruminal Fermentation and Gas Production
Format: Poster
Day/Time: Wednesday 7:30 AM–9:30 AM
Location: Exhibit Hall A
# W160
Fermentation of total mixed ration using microbial inoculants and its effect on in vitro rumen fermentation and microbial population in Hanwoo cows.
M. A. Miguel*1, L. L. Mamuad1, C. D. Jeong1, S. M. Kwon1, M. J. Ku1, Y. S. Choi2, Y. I. Cho1, S. S. Lee1, 1Department of Animal Science and Technology, College of Bio-industry Science, Sunchon National University, Suncheon, Jeonnam, Republic of Korea, 2Livestock Research Institute, Jeonnam Agricultural Research and Extension Services, Gangjin, Jeonnam, Republic of Korea.
Key Words: Hanwoo cow, rumen fermentation, total mixed ration
Fermentation of total mixed ration using microbial inoculants and its effect on in vitro rumen fermentation and microbial population in Hanwoo cows.
M. A. Miguel*1, L. L. Mamuad1, C. D. Jeong1, S. M. Kwon1, M. J. Ku1, Y. S. Choi2, Y. I. Cho1, S. S. Lee1, 1Department of Animal Science and Technology, College of Bio-industry Science, Sunchon National University, Suncheon, Jeonnam, Republic of Korea, 2Livestock Research Institute, Jeonnam Agricultural Research and Extension Services, Gangjin, Jeonnam, Republic of Korea.
This study determined the effect of microbial inoculants in fermenting total mixed ration (TMR) and used for in vitro rumen fermentation and microbial population in Hanwoo cows. Three different TMR were used in the study and each TMR were grouped into 3: non-fermented TMR (no inoculants) and fermented TMR (FTMR) for 7 and 14 d (inoculated with mixed culture of L. acidophilus and B. subtilis, 1.0 × 106 cfu/mL of each inoculant). After fermentation, the treatments were used for in vitro rumen fermentation using ruminal fluid collected from 3 cannulated Hanwoo cows.VFA was analyzed using HPLC and microbial population was determined by quantitative real-time PCR using target specific primers. The data were analyzed as a 3x3 factorial arrangement in a completely randomized design using the MIXED procedure of SAS. Fermentation quality of FTMR showed higher (P < 0.05) contents of lactate, acetate, NH3-N but lower (P < 0.05) pH, propionate and butyrate compared with the non-fermented TMR group. For the in vitro rumen fermentation, higher (P < 0.05) total gas production and NH3-N concentration were observed in the FTMR group. Specifically, FTMR2 and FTMR3 for 14 d had the highest (P < 0.05) total gas production at 6 and 24 h, whereas FTMR3 for 14 d had the highest (P < 0.05) NH3-N concentration at 24 h. Acetate, propionate and total VFA concentrations in the FTMR group were higher (P < 0.05) compared with non-fermented TMR group while no significant differences were observed in the butyrate production in all TMR groups. No differences were found in the number of total bacteria, Lactobacillus spp., Bacillus spp., and Fibrobacter succinogenes in all TMR groups. However, the number of protozoa increased (P < 0.05) whereas general anaerobic fungi and Ruminococcus flavefaciens population decreased (P < 0.05) in the TMR group fermented for 14 d. Overall, the results suggested that fermentation improved the quality of TMR and fermented total mixed ration can enhance rumen fermentation parameters and enable changes in the population of rumen microbes.
Key Words: Hanwoo cow, rumen fermentation, total mixed ration
