Nanofiltration (NF) is typically utilized during MPC manufacture to concentrate ultrafiltration retentate before drying. In related research we determined that increasing the NF temperature from 22°C to 50°C and use of hydrodynamic cavitation (HC) improved nanofiltration performance and increased the level of total solids that can be achieved. In this study 3 replicates of 4 different NF retentates were spray dried and the functionality of the dried MPC was evaluated. The NF treatments utilized were: NF at 22°C (NF22); NF at 50°C (NF50); HC before NF at 22°C (HCNF22); and HC before NF at 50°C (HCNF50). All samples were spray dried using a pilot scale dryer (Niro dryer Model 1, Niro Inc., Columbia) using air atomizing-flat spray nozzle (SUE15A, external mix) with an inlet and outlet temperature 170°C and 85°C respectively. The powders produced were analyzed for physicochemical and functional quality. The moisture content of all powders was below 5% and the protein and ash content ranged from 79.81 to 81.29% and 7.23–7.35% respectively. The loose bulk density was 353, 332, 358, and 323 Kg/m³ and the tapped bulk density was 454, 428, 456, and 421Kg/m³ respectively for the NF22, NF50, HCNF22, and HCNF50 treatments. The loose and tapped bulk density was significantly (P < 0.05) higher in treatments NF at 22°C, whereas HC did not have a significant (P > 0.05) impact on loose or tapped bulk density. There were no significant differences in solubility at 22°C or 50°C or wetting time at 22°C; however, HC significantly (P < 0.05) decreased the wetting time at 50°C. There were no significant (P > 0.05) differences in emulsification capacity, emulsion stability, or foam stability, whereas foam capacity was significantly higher in the treatments that were nanofiltered at 50°C. The rennet coagulation time significantly (P < 0.05) increased and the heat coagulation time significantly (P < 0.05) decreased in the treatments that were nanofiltered at 50°C. This study determined that NF temperature and HC have important effects on the functionality of dried MPC80 and can be utilized to adjust the functional characteristics of MPC80.