A study was conducted to evaluate the effects of feeding a palmitic enriched supplement on production responses of mid-lactating Jersey and Holstein cows. Eighty cows (40 Holstein and 40 Jersey) were used in a randomized complete block design with a split-plot arrangement, where the main plot was breed and subplot treatment. Cows within each breed were assigned to 1 of 2 treatments: 1) control diet without fat supplement, or 2) control diet plus a palmitic acid enriched supplement fed at 1.5% of diet dry matter. Cows were milked in 2 robotic milking units and treatments were offered through the robots. The diets contained 18.0% forage NDF, 29% NDF, 22.4% starch and 17.3% CP. Treatment period was 6 weeks with the final 3 weeks used for data and sample collection. The statistical model included the random effect of block and cow nested within breed, and the fixed effect of treatment, breed, time and their interactions. Preliminary milk yield was used as a covariate. Differences were declared at P ≤ 0.05 and tendencies at P ≤ 0.10. Compared with control, palmitic acid increased milk fat yield (1.36 vs. 1.27 kg/d; SEM = 0.06; P = 0.03), and tended to increase FCM (35.6 vs. 33.8 kg/d; SEM = 1.86, P = 0.09) as well as ECM (35.8 vs. 34.2 kg/d; SEM = 1.82, P = 0.10). There were no effects of palmitic acid on milk yield (P = 0.68), milk protein yield (P = 0.22), milk lactose yield (P = 0.74), BCS (P = 0.76) or BW change (P = 0.91). Compared with Holstein cows, Jersey cows had lower milk production (29.6 vs. 32.7 kg/d; SEM = 1.95, P = 0.02) and milk lactose yield (1.58 vs. 1.44 kg/d; SEM = 0.10, P = 0.04). Jersey cows tended to have greater milk fat yield (1.37 vs. 1.27 kg/d; SEM = 0.06, P = 0.10) compared with Holstein cows. There was a breed effect on BW change (P = 0.03); Holstein cows gained 0.385 kg/d during the experimental period, while Jersey cows gained 0.145 kg/d. There were no effects of breed on milk protein yield (P = 0.85), FCM (P = 0.97), ECM (P = 0.99), and BCS (P = 0.59). No interaction treatment × breed was detected for the variables analyzed. Overall, feeding palmitic acid enriched supplement increased milk fat yield on both Jersey and Holstein cows, and the response tended to be better in Jersey than Holstein cows.