Abstract #T158

# T158
Evaluation of supplemental autolyzed yeast on the presence of inflammatory biomarkers from Holstein cows fed a high starch diet.
S. E. Knollinger*1, B. Miller2, I. Mueller3, F. C. Cardoso1, 1University of Illinois, Urbana, IL, 2BIOMIN America Inc, Overland Park, KS, 3BIOMIN Holding GmbH, Getzersdorf, Austria.

The aim of this study was to investigate an AY (Saccharomyces cerevisiae) supplemented in high starch lactation diets and its effect on blood metabolites, in particular inflammatory biomarkers. Fifteen rumen-cannulated Holstein cows were assigned to 1 of 5 treatments in a replicated 5 × 5 Latin square design balanced to measure carryover effects. Treatments were: low starch diet without AY (LS0; control), high starch diet without AY (HS0), high starch diet with either 15 g (HS15), 30 g (HS30), or 45 g (HS45) of AY supplementation. The period of 21 d was divided into the adaptation phase (d 1 to 14) and a measurement phase (d 15 to 21). Blood was sampled from the coccygeal artery or vein at 0600 h on d 15, 18, and 21(n = 3) of each period from each cow. Data collected were analyzed using the MIXED procedure of SAS. Orthogonal contrasts were used. Contrasts; CONT1 = LS0 compared with HS0; CONT2 = HS0 compared with the average of the 3 AY treatments (HS0, HS15, HS45) and linear and quadratic treatment effects (HS0, HS15, HS30, and HS45). NEFA (93.69 vs. 82.71 ± 4.08 μEq/L; P = 0.04) was greater in cows fed LS0; insulin (0.93 vs. 0.74 ± 0.10 μg/L; P = 0.04) concentration was greater in cows fed HS0. Plasma gamma-glutamyl transferase (GGT; 26.09 vs. 24.94 ± 3.57 U/L; P = 0.05), and mineral phosphorus (5.86 vs. 5.38 ± 0.15 mg/dL; P = 0.005) concentrations were greater in HS0 compared with LS0. However, the LS0 diet had greater total protein (7.60 vs. 7.43 ± 0.42 g/dL; P = 0.03), and bilirubin (0.14 vs. 0.12 ± 0.008 mg/dL; P = 0.03) when compared with cows fed HS0. Supplementing AY to cows had greater plasma total protein (P = 0.008), globulin (P = 0.008), serum amyloid A (SAA; P = 0.02), but lower albumin:globulin (P = 0.03), and tendency for lower superoxide dismutase activity (SOD; P = 0.07; CONT2). Linear treatments effects for plasma BHB (0.52 ± 0.03 mmol/L; P = 0.08) was higher at HS15. In conclusion, cows in HS0 had lower insulin, total bilirubin and protein compared with LS0 but greater concentrations of albumin:globulin, and tendency for SOD compared with treatments with added AY (CONT2).

Key Words: high starch, yeast, inflammation