The addition of autolyzed yeast (AY) has proven to have beneficial effects on high starch diets resulting to positive implications on production parameters. This study postulated that the addition of AY (Saccharomyces cerevisiae) supplementation in a high starch lactation diet would improve milk production. Fifteen rumen-cannulated Holstein cows were assigned to 1 of 5 treatments in a replicated 5 × 5 Latin square design balanced to measure carryover effects. Treatments were: low starch diet without AY (LS0; control), high starch diet without AY (HS0), high starch diet with either 15 g (HS15), 30 g (HS30), or 45 g (HS45) of AY supplementation. The period of 21 d was divided into the adaptation phase (d 1 to 14) and a measurement phase (d 15 to 21). Cows were milked 3 times daily at 0400, 1200, and 1930 h. Milk weights were recorded at every milking during the measurement phase and milk samples were obtained at each milking on d 15 and 20 of each period. Data were analyzed using the MIXED procedure of SAS. Cows in HS0 had increased DMI (24.91 vs. 19.93 kg/d; P < 0.0001), BW (689 vs. 665 kg; P = 0.003), milk yield (34.51 vs. 30.50 kg/d; P = 0.0006), and ECM (34.39 vs. 31.27 kg/d; P = 0.03) compared with cows in LS0. Cows in HS0 had greater true protein (1.10 vs. 0.94 kg/d; P = 0.0008), casein (0.43 vs. 0.32 kg/d; P = 0.002), and lactose (1.63 vs. 1.41 kg/d; P = 0.004) yields compared with cows in LS0. In relation to the HS0 treatment, cows in LS0 had greater fat concentration (3.89 vs. 3.56%; P = 0.007), and MUN (14.37 vs. 13.56 mg/dL; P = 0.09). The LS0 treatment had greater FCM/DMI (1.62 vs. 1.32; P = 0.0003), ECM/DMI (1.64 vs. 1.32; P < 0.0001), and milk/DMI (1.56 vs. 1.38; P = 0.008) efficiencies when compared with cows in HS0. Cows in HS45 had increased DMI (24.91 vs. 25.59 kg/d; P = 0.02) than cows in HS0. Cows in HS15 tended to improve 3.5% FCM/DMI (1.42 vs. 1.32; P = 0.09), and ECM/DMI (1.41 vs. 1.32; P = 0.07) efficiencies when compared with cows in HS0. As expected, cows receiving a high starch diet had increased DMI, ECM, and milk yield than cows in the low starch diet. In conclusion, adding AY improved DMI and production efficiencies.