Abstract #T52
Section: Dairy Foods (posters)
Session: Dairy Foods - Probiotics, Bioactives and Health
Format: Poster
Day/Time: Tuesday 7:30 AM–9:30 AM
Location: Exhibit Hall A
Session: Dairy Foods - Probiotics, Bioactives and Health
Format: Poster
Day/Time: Tuesday 7:30 AM–9:30 AM
Location: Exhibit Hall A
# T52
Investigation and comparison of the anti-tumor effects of lactoferrin, α-lactalbumin, and β-lactoglobulin in A549, HT29, HepG2, and MDA231-LM2 models.
H. Li1,2, P. Li1,2, H. Yang1,2, Y. Wang1,2, G. Huang1,2, J. Wang*1,2, N. Zheng1,2, 1State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China, 2Key Laboratory of Quality & Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
Key Words: lactoprotein, antitumor activity, tumor-bearing model
Investigation and comparison of the anti-tumor effects of lactoferrin, α-lactalbumin, and β-lactoglobulin in A549, HT29, HepG2, and MDA231-LM2 models.
H. Li1,2, P. Li1,2, H. Yang1,2, Y. Wang1,2, G. Huang1,2, J. Wang*1,2, N. Zheng1,2, 1State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China, 2Key Laboratory of Quality & Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
To investigate the anti-tumor activities of lactoferrin, α-lactalbumin, and β-lactoglobulin, 4 kinds of human tumor cells (lung tumor cell A549, intestinal epithelial tumor cell HT29, hepatocellular cell HepG2 and breast cancer cell MDA231-LM2) were exposed to 3 proteins, respectively. The effects on cell proliferation, migration, and apoptosis were detected in vitro, and nude mice bearing tumors were administered with 3 proteins in vivo. Results showed that 3 proteins (20 g/L) inhibited viability and migration, as well as induced apoptosis, in 4 tumor cells with different degrees (compared with the control, P < 0.05). In vivo, tumor weights in HT29 group (0.84 ± 0.22 g v.s. control 2.05 ± 0.49 g) and MDA231-LM2 group (1.11 ± 0.25 g v.s. control 2.49 ± 0.57 g) were significantly reduced by lactoferrin (P < 0.05); tumor weights in A549 group (1.07 ± 0.19 g v.s. control 3.11 ± 0.73 g) and HepG2 group (2.32 ± 0.46 g v.s. control 3.50 ± 0.74 g) were significantly reduced by α-lactalbumin (P < 0.05). Moreover, the roles of lactoferrin, α-lactalbumin, and β-lactoglobulin in regulating apoptotic proteins were validated. In summary, lactoferrin, α-lactalbumin, and β-lactoglobulin were proved to inhibit growth and development of A549, HT29, HepG2, MDA231-LM2 tumors in different degrees, via induction of cell apoptosis.
Key Words: lactoprotein, antitumor activity, tumor-bearing model
