Abstract #T20
Section: Animal Health (posters)
Session: Animal Health Posters 2
Format: Poster
Day/Time: Tuesday 7:30 AM–9:30 AM
Location: Exhibit Hall A
Session: Animal Health Posters 2
Format: Poster
Day/Time: Tuesday 7:30 AM–9:30 AM
Location: Exhibit Hall A
# T20
Mitigating environmental mastitis microbes with the novel use of paper mill byproducts.
D. C. Reyes*1, S. A. Rivera1, Z. X. Ma2, H. M. Dubuc1, A. Y. Leon-Tinoco1, A. B. Lichtenwalner1, A. Bryant1, D. P. Marcinkowski1, K. C. Jeong2, J. J. Romero1, 1Animal and Veterinary Sciences, School of Food and Agriculture, University of Maine, Orono, ME, 2Department of Animal Sciences, University of Florida, Gainesville, FL.
Key Words: technical lignins, mastitis, antimicrobial
Mitigating environmental mastitis microbes with the novel use of paper mill byproducts.
D. C. Reyes*1, S. A. Rivera1, Z. X. Ma2, H. M. Dubuc1, A. Y. Leon-Tinoco1, A. B. Lichtenwalner1, A. Bryant1, D. P. Marcinkowski1, K. C. Jeong2, J. J. Romero1, 1Animal and Veterinary Sciences, School of Food and Agriculture, University of Maine, Orono, ME, 2Department of Animal Sciences, University of Florida, Gainesville, FL.
To identify potential bedding conditioners for prevention of mastitis in dairy cattle, we determined the minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) of a set of technical lignins. A broth macrodilution assay using Mueller Hinton media modified to pH 6 was used to evaluate antimicrobial activity against strains of Streptococcus uberis, Staphylococcus hyicus, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa isolated from mastitic and metritic cows. Technical lignin treatments (TRT) evaluated were: 1) alkali Kraft lignin (AKL), 2) sodium lignosulfonate (NaL), 3) magnesium lignosulfonate (MgL), and 4) washed Kraft lignin (WKL). The MIC and MBC assays were carried out in duplicate in each of 3 independent runs. In this study, gram-positive bacteria (S. hyicus and S. uberis) were more susceptible to the antimicrobial activity of NaL compared with gram-negative bacteria, with E. coli being the most resistant (Table 1). AKL was the most effective lignin against K. pneumoniae, but its activity against all the other bacteria was limited compared with NaL. Except for K. pneumoniae, MgL required a much higher concentration to kill bacteria relative to the dose that inhibits growth. In the case of NaL, this large difference was observed only against K. pneumoniae. Overall, NaL had the broadest antimicrobial spectrum against the bacteria tested and has potential to be a novel bedding conditioner that can help to control environmental mastitis pathogens in stalls.
Table 1 (Abstr. T20). MIC and MBC values (mg/mL ± SD) for technical lignins against bacteria isolated from mastitic and metritic cows
1Not available.
2Could not be determined visually.
| Bacteria | TRT | ||||
| NaL | MgL | AKL | WKL | ||
| E. coli | MIC | 27.5 ± 5.0 | 20.0 ± 0 | >60 | >60 |
| MBC | 30.0 ± 8.2 | 56.7 ± 5.77 | n.a.1 | n.a. | |
| S. hyicus | MIC | 6.25 ± 2.5 | 25.0 ± 5.77 | 16.6 ± 11.5 | >60 |
| MBC | 8.75 ± 0.6 | 30.0 ± 4.8 | 50.0 ± 17.3 | n.a. | |
| S. uberis | MIC | 5.8 ± 1.4 | 23.3 ± 5.77 | 10.0 ± 0 | >60 |
| MBC | 5.8 ± 1.4 | 30.0 ± 0 | >60 | n.a. | |
| P. aeruginosa | MIC | 16.6 ± 5.77 | 26.6 ± 11.5 | >60 | >60 |
| MBC | 20.0 ± 0 | 40.0 ± 0 | n.a. | n.a. | |
| K. pneumoniae | MIC | 8.33 ± 2.88 | 10.0 ± 0 | 2.5 ± 0 | n.d.2 |
| MBC | 40.0 ± 0 | 10.0 ± 0 | 4.37 ± 1.25 | 56.7 ± 1.25 | |
Key Words: technical lignins, mastitis, antimicrobial
