Abstract #T113

# T113
Meta-analysis of the effect of Lactobacillus buchneri inoculation on dry matter recovery and aerobic stability of silages.
Kathy G. Arriola*1, Andre S. Oliveira2, Yun Jiang1, Ibukun M. Ogunade1, Donghyeon Kim1, Henrique M. Silva2, Felipe X. Amaro1, Andres A. Pech-Cervantes1, Sam C. Kim3, Halima Sultana1, Diwakar Vyas1, Luiz F. Ferraretto1, Adegbola T. Adesogan1, 1Department of Animal Sciences, University of Florida, Gainesville, FL, 2Institute of Agriculture and Environmental Sciences, Universidade Federal de Mato Grosso, Sinop, Brazil, 3Division of Applied Life Science, Gyeongsang National University, Jinju, South Korea.

A meta-analysis of 103 peer-reviewed papers was conducted to examine effects of inoculation with Lactobacillus buchneri (LB) or homolactic and or facultative heterolactic acid bacteria (HAB) in combination with LB (LB+HAB) on silage DM recovery and aerobic stability. Raw mean differences (RMD) between inoculant and control treatment means that had been weighted by inverse variance using random models were compared. Heterogeneity sources evaluated included forage type, application rate (AR; < 104, 105, 106 or >107 cfu/g as fed, bacteria type (LB vs. LB plus each of various HAB), enzyme inclusion, ensiling duration and silo type (laboratory or farm-scale) as covariates. Inoculation increased aerobic stability (RMD = 95 ± 52h, P < 0.01, n = 163) and DM recovery (RMD = 0.81 ± 0.75%, P = 0.04, n = 112) but high heterogeneity was detected (I2 statistic = 99.8 and 98.3%, respectively). Enzyme use, ensiling duration or silo type did not affect aerobic stability or DM recovery and AR did not affect aerobic stability. Inoculation did not affect the aerobic stability of silages of tropical grasses (RMD = 12 ± 21 h, n = 6), but tended to increase those of corn (RMD = 84 ± 61 h, P = 0.06, n = 66), temperate grasses (RMD = 102 ± 18 h, P < 0.01, n = 44), alfalfa (RMD = 71 ± 26 h, P < 0.01, n = 3), other legumes (RMD = 24 ± 8 h, P < 0.01, n = 5), high moisture grain (RMD = 174 ± 35 h, P < 0.01, n = 17) and other crops (RMD = 61 ± 46 h, P < 0.04, n = 22). The LB and LB+HAB types similarly increased aerobic stability (P > 0.10), except LB plus L. plantarum (LP) and Enterococcus faecium (EF), which had no effect (RMD = 17 ± 32 h, P = 0.31, n = 22). Forage or bacteria type did not affect DM recovery, but unlike higher doses, inoculation with 105 cfu/g increased DM recovery (RMD = 1.22 ± 0.66%, P < 0.01, n = 88). Inoculation with LB alone or combined with HAB (except LB+LP+EF) markedly improved the aerobic stability of corn, alfalfa and other legumes, temperate grasses and high moisture grain, but not tropical grasses. Inoculation also increased DM recovery when applied at 105 cfu/g but not at higher doses.

Key Words: Lactobacillus buchneri, aerobic stability, silage