Abstract #T112

# T112
Effect of bacterial inoculants containing Lactobacillus buchneri and/or Lactobacillus hilgardii on the fermentation and quality of corn silage.
Kathy G. Arriola*1, Diwakar Vyas1, Donghyeon Kim1, Mariele C. Agarussi1, Vanessa P. Silva1, Juan M. Flores1, Yun Jiang1, Andres A. Pech-Cervantes1, Adegbola T. Adesogan1, 1Department of Animal Sciences, University of Florida, Gainesville, FL.

The objective was to determine the effect of Lactobacillus buchneri and/or Lactobacillus hilgardii inoculation on the fermentation and chemical composition of corn silage. Two corn hybrids harvested at either 34 or 43.8% DM (Experiment 1 or 2) were chopped and ensiled in vacuum-sealed bags (40 × 61cm) for 30 and 90 d. Treatments included (1) deionized water (CON) or inoculants containing (2) Lactobacillus buchneri (1.5 × 105 cfu/g, LB); (3) Lactobacillus hilgardii (1.5 × 105 cfu/g; LH); and (4) Lactobacillus buchneri and L. hilgardii (1.5 × 105 cfu/g of each inoculant, LB+LH). Samples collected from d 0, 30, and 90 were analyzed for chemical composition and microbial counts. Aerobic stability was measured at d 30 and 90. Data for each experiment were analyzed separately with the Glimmix procedure of SAS for a completely randomized design. In Experiment 1 (34% DM) d 30 samples, inoculation tended to reduce lactate to acetate ratio (6.5 vs. 6.9; P = 0.07) and increase aerobic stability (301 vs. 197 h; P = 0.07) and LH and LB+LH reduced the pH compared with CON. In d 90 samples, inoculation increased aerobic stability (488 vs. 400 h) and LAB counts compared with CON (7.93 vs. 6.34 log cfu/g; P < 0.0001). Also, LH reduced yeast counts relative to CON and LB+LH reduced lactate concentration relative to CON and LB. In Experiment 2 (43.8% DM) d 30 samples, LH reduced DM loss relative to LB (1.89 vs. 9.07%) and LB increased acetate relative to other treatments; inoculation increased (P < 0.0001) LAB counts, and reduced lactate concentration (tendency) and yeast counts compared with CON. In d 90 samples, inoculation increased LAB counts (8.91 vs. 6.96 log cfu/g; P < 0.0001) and reduced yeast counts (0 vs. 2.38 log cfu/g; P = 0.003) compared with CON. In conclusion, inoculating a corn hybrid harvested at 34% with LB, LH, and LB+LH improved LAB counts and aerobic stability after 30 or 90d of ensiling. However, inoculating a hybrid harvested at 43.8% DM improved LAB counts and reduced yeast counts at d 30 and 90 but did not affect aerobic stability.

Key Words: corn silage, Lactobacillus buchneri, Lactobacillus hilgardii