Abstract #T283

# T283
Effects of close-up dietary energy level and supplementing rumen protected lysine on blood β-hydroxybutyrate concentration and milk production in transition cow.
Girma Debele Delelesse1, Lu Ma1,2, Fang Wang1, Qingrong Jiang1, Dengpan Bu*1,3, 1Institute of Animal Science, State key Laboratory of Animal Nutrition, Chinese Academy of Agricultural Sciences, Beijing, China, 2CAAS-ICRAF Joint Lab on Agroforestry and Sustainable Animal Husbandry, World Agroforestry Centre, East and Central Asia, Beijing, China, 3Hunan Co-Innovation Center of Safety Animal Production, Changsha, Hunan, China.

The objective of this study was to investigate the close-up dietary energy level and supplementing of rumen-protected lysine on blood β-hydroxybutyrate level, dry matter intake, milk production and composition in transition cows. Sixty-eight multiparous Holstein cows were allocated to 4 treatments arrange in a 2 × 2 factorial arrangement with dietary NEL level (1.37 Mcal/kg vs 1.53 Mcal/kg: HE vs LE) and addition of rumen protected lysine (from Ascor ChimiciSrl, 40 g or 0 g: Blys or CK) as 2 factors. All cows were randomly allocated into 4 treatment groups (HEBlys, HECK, LEBlys and LECK) after balanced in their expected calving date, previous milk yield and body condition score. All cows were fed with the same NEL (1.34 Mcal/kg DM) diet in dry off period. Rumen protected-lysine was top dressed at 40 g/d/cow to total mixed ration 3 weeks before to 3 weeks after calving. After calving all cows received the same NEL (1.69 Mcal/kg DM) diet. Blood samples were collected at −21, −14, −7, 0, 3, 7, 14 and 21 d relative to calving and blood β-hydroxybutyrate concentration was measured. Amount of feeds offered and refused for individual cows were collected and measured 4 d per week. Milk samples were collected once per week and analyzed for milk composition. Data were analyzed using proc mixed SAS (SAS institute, Inc., Cary NC). Feeding high NEL to close up cows decreased the concentration of β hydroxybutyrate (0.55 mol/mL vs 0.37 mol/mL, P < 0.0001) in pre-partum cows. Rumen protected lysine improved postpartum dry matter intake (18.9 kg/d vs 19.5 kg/d, P < 0.0001) and decreased β-hydroxybutyrate concentration (1.62 mol/mL vs 0.95 mol/mL, P < 0.01). Either energy or bypass lysine and their interaction did not affect milk yield, and percentage of milk fat and protein. Cows fed HE together with Bypass lysine reduced in β-hydroxybutyrate concentration (1.09 mol/mL vs 0.81 mol/mL, P < 0.02) in postpartum cows and tended to produce more milk. This revealed that feeding high-energy diet together with rumen protected lysine favorable in improving dry matter intake and lower β-hydroxybutyrate levels in transition cow.

Key Words: β-hydroxybutyrate, rumen-protected lysine, transition cow