Approximately 70% to 75% of the world’s adult population cannot fully utilize dairy due to varying degrees of lactase deficiency. With the high incidence of lactose intolerance in the population there is a need for reliable utilization of oral lactase enzyme. In a 2010 literature review, the effectiveness of prehydrolyzed milk products or lactase was questioned. Problems with study design, source of enzyme, and dosage were cited. The aim of our study was to explore the effects of pH and temperature on lactase kinetics to determine a more effective delivery method. Four antacids were investigated as modifiers of simulated stomach acid. The effects of antacid pretreatment on lactase preservation in acid (HCl) were evaluated at 4°C and 37°C using a spectrophotometric assay. This assay measured the appearance of o-nitrophenol from the hydrolysis of o-nitrophenyl-β-galactoside (ONPG) as a substrate. Using the same model, data from simultaneous addition of antacid with lactase to an acidic solution were generated. Glucose production from lactose was monitored with a handheld glucometer to determine the effectiveness of exogenous lactase under varying in vitro conditions. Three of 4 antacids were found to raise the pH of an acidic medium to within a sufficient working range (approx. 5.5–7.5) at all tested volumes. Sodium bicarbonate (NaHCO₃) was observed to neutralize acid fastest. Lactase enzyme was found to hydrolyze lactose approximately 1.6 times faster at 37°C compared with 4°C. Using an ONPG assay, NaHCO₃-containing antacids proved effective at enzyme preservation when added to acid simultaneously with lactase. When added to 100mL of 0.1M HCl, a combined lactase/NaHCO₃ preparation in whole milk (240mL) yielded glucose concentrations of 202 ± 23.5 mg/dL at 5mins and 520 ± 30.7 mg/dL at 10 min. In contrast, an antacid-free preparation yielded values less than the minimum detectable concentration of the glucose meter, 20 mg/dL. In our pursuit to find an effective lactase preparation and delivery method, we suggest a lactase-sodium bicarbonate pairing to allow for immediate lactase-lactose interaction within the stomach while still avoiding any acid-mediated enzyme degradation.