Detection of standing heat is difficult for cattle in tiestall barns and many farmers use secondary signs of estrus behavior including bellowing and vaginal discharge for AI. The aim of this study was to determine pregnancy success to AI after detection of estrus in cattle in tie-stall barns using by secondary signs. The study was performed using reproductive data from 175 cattle of Thai-Holstein dairy cattle in tiestall barns in Chiang Mai Province, Thailand, during February to July 2017. After observing secondary signs of estrus, farmers notified the researchers who performed AI within 12, 24, or 36 h after estrus exhibition. Data on season (cool, summer, rainy), signs of estrus (bellowing, vaginal discharge, redness of vulva, swollen vulva), duration between observed estrus and AI time (12, 24, 36 h) were independent variables. Pregnancy checks performed at 60 d after AI was defined as a dependent variable. Multiple logistic regressions were used to determine the factor relating to pregnancy. Results showed that cattle with combination of bellowing and having vaginal discharge had the highest pregnancy rate (68%) and significantly higher than cattle with other signs (OR = 2.02). The pregnancy rate in cool and summer seasons were higher than rainy season with OR = 3.67 and OR = 1.63, respectively. Cattle inseminated at 24 h after expression had significantly higher pregnancy rate than 12 h (OR = 2.67). Results indicated that highest pregnancy rates were achieved with cattle displaying bellowing and vaginal discharge, with insemination interval of 24 h after signs of estrus, and in the cool season.