Abstract #T277
Section: Ruminant Nutrition (posters)
Session: Ruminant Nutrition II
Format: Poster
Day/Time: Tuesday 7:30 AM–9:30 AM
Location: Exhibit Hall A
Session: Ruminant Nutrition II
Format: Poster
Day/Time: Tuesday 7:30 AM–9:30 AM
Location: Exhibit Hall A
# T277
In vitro assessment of oil releasing extent from calcium salt of fat supplements in different sites of gastrointestinal tract.
Peyman Peravian1, Mehdi Dehghan Banadaky*2, Hamidreza Mirzai3, Pedram Rezamand4, Hamed Khalilvandi5, 1University of Tehran, Tehran, Tehran, Iran, 2College of Agriculture and Natural Science, University of Tehran, Alborz, Karaj, Iran, 3Zanjan University, Zanjan, Zanjan, Iran, 4Animal and Veterinary Science Department, University of Idaho, Moscow, ID, 5University of Urmia, Urmia, Urmia, Iran.
Key Words: gastrointestinal tract, calcium salt of fatty acid, oil releasing extent
In vitro assessment of oil releasing extent from calcium salt of fat supplements in different sites of gastrointestinal tract.
Peyman Peravian1, Mehdi Dehghan Banadaky*2, Hamidreza Mirzai3, Pedram Rezamand4, Hamed Khalilvandi5, 1University of Tehran, Tehran, Tehran, Iran, 2College of Agriculture and Natural Science, University of Tehran, Alborz, Karaj, Iran, 3Zanjan University, Zanjan, Zanjan, Iran, 4Animal and Veterinary Science Department, University of Idaho, Moscow, ID, 5University of Urmia, Urmia, Urmia, Iran.
The purpose of this study was the evaluation of oil releasing extent (ORE) in fat calcium salt supplements (Persiafat) in simulated culture of gastrointestinal tract (GT) < rumen, abomasum, small intestine (SI) >. Treatments included 1.5 g fish oil, 1.5 g flaxseed oil, 1.5 g blended oil, 1.5 g mixture of fish and flaxseed oil. Rumen fluid (RF) was collected from fistulated cow 4 h after feeding. Anaerobic environment was provided by RF filteration in textile and using CO2 gas. Six bottles were used for incubation in each site of GT. 1.5 g fat calcium salt and 50 mL rumen fluid were added in each bottle, pH was adjusted to 6.4 and incubation was done in 39°c with 100 rpm for 24 h in batch culture. Bottle components were filtered in textile after incubation and fat extraction was done by Folch (chloroform/methanol (2/1)) method. ORE in abomasum and SI was measured by preparing 3.2 g pepsin (pH = 1.2) and 10 g pancreatin (pH = 6.8) with specific solution for abomasum and SI in order. Incubation time was 3 h for both abomasum and SI. The steps of measuring ORE was done according to rumen but petroleum solution was utilized rather than Folch. The results showed that percent of oil releasing between treatments in rumen were significant (P < 0.01) (15.64, 19.93, 14.8, 11.11 for treatment 1–4 respectively), however the highest was for flaxseed and the lowest was for blended oil. As it was expected, low pH in abomasum dissociated the linkage between calcium and fatty acid so the highest ORE was in abomasum in compare of other sites of GT (90% < ) and the percent of oil releasing between treatments were not significant (P > 0.05). (87.02, 93.79, 94.84, 94.40 for treatment 1–4 respectively) .In small intestine treatments affect ORE (P < 0.01) (6.84, 8.14, 5.17, 7.99 for treatment 1–4 respectively) and the highest was for flaxseed and the lowest was for fish oil. According to results of this study, calcium salt of fatty acid could be used with minimum effects on rumen fermentation because lowest oil releasing happend in rumen in compare of other protection methods.
Key Words: gastrointestinal tract, calcium salt of fatty acid, oil releasing extent
