The use of co-products as an alternative feed source is a common practice when formulating dairy rations. A study using 12 multiparous (79 ± 16 DIM; mean ± SD) lactating Jersey cows, was conducted to evaluate the effects of dried distillers grains with solubles (DDGS) or canola meal (CM) on milk and methane production. A replicated 4 × 4 Latin square design was used to compare 4 different dietary treatments over 4 periods. Each of the 4 periods utilized 23 d for adaptation with 5 d of collection. Treatments were composed of a control (CON) containing corn and soybean meal and no co-products, a treatment diet containing 10% (DM basis) DDGS (DDGS),10% DDGS treatment with an alternative distillers grains source (ADDGS), and a 10% canola meal (CAN) treatment. Co-products were included in partial replacement for corn and soybean meal. Indirect calorimeters were used to sample methane. Dry matter intake and milk yield were similar (P > 0.55) between all treatments averaging 17.5 ± 0.78 kg/d and 24.1 ± 0.80 kg. Milk protein and fat percentage was similar across treatments (P ≥ 0.43) averaging 3.64 ± 0.04% and 6.18 ± 0.17%, respectively. Compared with CON, the addition of DDGS, CAN, or ADDGS did not affect total methane produced (P = 0.54) averaging 340.2 ± 19.59 L/d. When expressing methane per unit of DMI, all treatments were similar (P = 0.75) averaging 19.6 ± 1.26 L/kg/d. Heat production per day of metabolic body weight tended to be lowest in CON (P = 0.06) followed by DDGS, ADDGS and CAN (203.83, 210.73, 215.23, 227.70 ± 6.27 heat production/d/MBW, respectively). Milk urea nitrogen (MUN) was also affected by treatment (P < 0.01), CON and CAN were not different (20.7 and 19.9 ± 0.62 mg/dl, respectively) and DDGS and ADDGS were not different (18.1 and 18.1 ± 0.62 mg/dl, respectively). Results of this study indicate that milk production; milk components and methane production are not adversely affected when feeding the current inclusion rate of common co-products replacing both corn and soybean meal.