Abstract #M293

Section: Ruminant Nutrition
Session: Ruminant Nutrition I
Format: Poster
Day/Time: Monday 7:30 AM–9:30 AM
Location: Exhibit Hall B

# M293
Calves fed with oregano and green tea extracts alter slightly their blood redox status.
V. Fischer^*1, M. de Paris¹, S. C. B. Stivanin¹, E. F. Vizzotto¹, M. B. Zanela², C. Klein¹, V. Stone¹, C. Matte¹, ¹Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil, ²Empresa Brasileira de Pesquisa Agropecuaria, Pelotas, RS, Brazil.

This work aimed to investigate the effect on antioxidant status in dairy calves fed milk with Origanum vulgare or Camellia sinensis extracts. Twenty-four calves were randomly assigned to one of the following treatments: control (CO, no plant extract), green tea extract (GTE, 30 mg/kg BW/day) and oregano extract (OE 60mg/kg BW/day) from birth to weaning. Calves received 4 L/d of milk from birth to 60 d of life when they were weaned. Plant extracts were added into the milk immediately before feeding. Blood samples were collected by jugular vein puncture on d 1, 30 and 60 of life and further analyzed for redox status parameters. Data were submitted to variance analysis, using the Mixed procedure of SAS according to a completely randomized design, considering the effect of diet (n = 3), period (n = 3), diet by period interaction, calves as random effect and days as repeated measures. Overall, calves of CO group showed higher values for glutathione peroxidase (GPx) activity than calves of GTE and OE groups. Calves of the OE group tended to show higher values for superoxide dismutase (SOD) activity than calves of GTE group. There were no differences for the remaining parameters evaluated between groups. On d 30 calves from groups CO tended to show higher values for dichlorofluorescein oxidation in the plasma (DCFP) than GTE and OE groups (2678, 2201, 2067 nmol/mg of protein, P < 0.10), but on d 60, these results were reversed as calves of CO group showed lower values for DCFP than calves of GTE and OE groups (2111, 3242, 3468 nmol/mg, P < 0.05). Calves fed milk with green tea or oregano extracts show little changes in the blood redox status. Table 1.

Item	Means (SE)
Item	Control	Green Tea	Oregano
SOD¹	26.2 ± 1.63^cd	23.3 ± 1.68^d	28.8 ± 1.86^c
CAT¹	3.45 ± 0.28^a	3.35 ± 0.29^a	3.31 ± 0.32^a
GPX²	9.84 ± 0.7^a	7.38 ± 0.7^b	7.03 ± 0.8^b
GSH ²	0.07 ± 0.02^a	0.095 ± 0.02^a	0.05 ± 0.02^a
Carbonyl²	1.93 ± 0.16^a	1.74 ± 0.16^a	2.02 ± 0.18^a
Thiols²	0.63 ± 0.03^a	0.66 ± 0.03^a	0.70 ± 0.04^a

^a,b or ^c,dMeans in the same row followed by different letters differ (Lsmeans, p < 0.05) or tend to differ (P < 0.10). ¹In U/mg. ²In nmol/mg.

Key Words: plant extract, pre-weaning calf, redox