Abstract #M229

# M229
Feeding green tea extracts has minor effects on antioxidant status of dairy cows during the transition period.
V. Fischer*1, S. C. B. Stivanin1, E. F. Vizzotto1, M. de Paris1, M. B. Zanela2, C. Matte1, C. Klein1, V. Stone1, 1Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil, 2Empresa Brasileira de Pesquisa Agropecuária, Pelotas, RS, Brazil.

This work aimed to investigate the effect on redox status in dairy cows fed Origanum vulgare or Camellia sinensis extracts during the transition period. Twenty-four Jersey cows with initial overall BW 430 ± 33kg were randomly assigned to one of the following treatments: control (CO, no plant extract), green tea extract (GTE, 5 g/cow/day) and oregano extract (OE, 10 g/cow/day). Cows received the plant extracts from −21 d to +21 d relative to parturition and were fed with the same basal diet, composed of corn silage, concentrate, and pasture. Blood samples were collected by jugular vein on d −21, −6, +2, and +21 relative to parturition and further analyzed for redox status parameters. Data were submitted to variance analysis, using the Mixed procedure of SAS according to a completely randomized design, considering the effect of diet (n = 3), day of measurement (n = 4), diet by day interaction, cows as the random effect and data collected at different days as repeated measures. Treatments did not affect milk production (overall mean 16.6 ± 3.3 L/d). Cows fed GTE had lower values for dichlorofluorescein oxidation in erythrocytes (DCFE) than cows of control and OE groups. There were no significant differences between groups for dichlorofluorescein oxidation in plasma (DCFP), carbonil, thiols contents as well as superoxide dismutase (SOD), catalase (CAT), gluthatione peroxidase (GPx) and reduced gluthatione activities. Cows fed with green tea extract reduced DCFE, suggesting a slight positive effect on blood redox status. Table 1.
ItemMeansSE
ControlGreen TeaOregano
DCFE112,246b10,288a16,433b719
DCFP14,006a3,929a3,749a380
Carbonil11.6a1.6a1.7a0.1
Thiols0.20a0.20a0.20a0.0
SOD116.3a15.3a17.3a0.7
CAT11.2a1.1a1.2a0.1
GPx20.8a0.7a0.8a0.1
GSH20.1a0.1a0.01a0.0
a,bMeans in the same row followed by different letters are significantly different (Lsmeans, P < 0.05). 1In nmol/mg of protein. 2In U/mg of protein.

Key Words: plant extract, dairy cows, redox status