Previous research has established that soluble soybean polysaccharide (SSPS) can enhance lactose crystallization in pure lactose solutions. However, commercial lactose is typically manufactured by crystallization of concentrated permeate (CP). The objective of this study was to determine the feasibility of using SSPS to improve lactose yield during manufacturing of lactose from CP. A laboratory scale crystallization set up with parallel crystallizers was utilized to conduct control and treatment (with SSPS) experiments simultaneously. CP (total solids from 58 to 60% and 48 to 49% Lactose) obtained from a lactose manufacturer was used in the experiments. CP was heated to 80°C to dissolve lactose before transferring to the crystallization tanks. The CP solution in the tanks was cooled from 80°C to 18°C (rate, −0.0479°C /min) using an automatic temperature controlled water batch. Constant agitation of 120 rpm was applied during the cooling cycle. Both the control and treatment solutions were seeded with lactose crystals (0.027 g/100 g of solution) and 0.1% SSPS was added to treatment solution. After completion of crystallization, chilled water (at 4°C, 15 g per 100 g of solution) was added to the crystallized solution and centrifuged at 10,000 × g for 20 min at 4°C. The supernatant was decanted, weighed and an equal quantity of deionized water (4°C) was added to wash the crystals. A total of 4 washing cycles were applied to purify the lactose crystals. The mass of the washed lactose crystals (corrected for total solids) was used to calculate lactose yield. The collected supernatant from each washing was freeze-dried and analyzed for SSPS. Lactose yield with 0.1% SSPS addition was significantly (P < 0.05) higher (76.1%) as compared with the control (71.5%). Out of the total SSPS added to the treatment solution, 79.7% was recovered in to the wash water. The findings of this study suggest potential feasibility of SSPS for enhancing lactose crystallization during lactose manufacture from concentrated permeate.