Our objective was to determine the effects of feeding oscillating dietary RDP levels on production and nitrogen (N) utilization in dairy cows. Eight Holstein cows were used in a replicated 4 × 4 Latin square design with 4 dietary treatments. Three diets were formulated to contain low (9.34% of DM), medium (11.3%), and high (12.6%) RDP levels. Dietary treatments were: 1) a diet containing 9.34% RDP fed on a continuous basis (LR); 2) a diet containing 11.3% RDP fed on a continuous basis (MR); 3) feeding the 9.34 and 11.3% RDP diets on an oscillating (48-h) basis (LRMR); and 4) feeding the 9.34 and 12.6% RDP diets on an oscillating basis (LRHR). Dry matter intake was greater (P < 0.01) in cows fed MR compared LRHR; however, milk yield was unaffected by diet. Milk fat content and yield were greater (P ≤ 0.05) in cows fed LRHR compared with MR. Milk urea-N content was greater (P < 0.01) in cows fed LRHR compared with LRMR. Nitrogen intake (NI) tended to be greater (P = 0.09) in cows fed LRHR compared with MR. Also, NI tended to be greater (P = 0.08) in cows fed LRHR compared with LRMR. Urine N excretion (g/d or % of NI) was greater (P ≤ 0.05) in cows fed LRHR compared with MR. Urine N excretion (g/d or % of NI) was greater (P ≤ 0.01) in cows fed LRHR compared with LRMR. Fecal N excretion (g/d or % of NI) was greater (P ≤ 0.01) in cows fed LRHR compared with MR. Fecal N excretion (% of NI) was greater (P = 0.01) in cows fed LRHR compared with MR. Total N excretion (g/d or % of NI) was greater (P ≤ 0.03) in cows fed LRHR compared with MR. Total N excretion (as g/d or % of NI) was greater (P ≤ 0.01) in cows fed LRHR compared with LRMR. Apparent N balance tended to be greater (P = 0.07) in cows fed static RDP diets compared with those fed oscillating RDP diets. Milk N secretion and productive N (i.e., apparent N balance + milk N) were unaffected by diet. Our results show that feeding oscillating RDP levels does not improve N utilization when compared with static RDP; however, feeding LRMR improved N utilization compared with LRHR.