Abstract #T87

Section: Dairy Foods
Session: Dairy Foods VI: Dairy Ingredients
Format: Poster
Day/Time: Tuesday 7:30 AM–9:30 AM
Location: Exhibit Hall B

# T87
Mycobiota and natural incidence of aflatoxin M1 in milk based dietary supplements.
B. Pereira^1,2, V. Farias¹, L. Luquez³, E. Rodrigues³, R. Franco¹, L. Keller^*1, ¹UFF, University Federal Fluminense, Niterói, RJ, Brazil, ²CAPES, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brasília, DF, Brazil, ³PESAGRO, Empresa de Pesquisa Agropecuária do Estado do Rio de Janeiro, Niterói, RJ, Brazil.

The actions and supervision of the Brazilian government to enforce the regulations to adapt to the new requirements, should raise the quality standards of the entire dairy chain. The objective of this study was to analyze the microbiota and natural incidence of aflatoxin M1 (AFM1) in milk based dietary supplements. For the analysis were used the standards described by the normative instruction n. 62 by Brazilian Ministry of Agriculture, Livestock and Supply (MAPA); the normative instruction no. 7 by National Health Surveillance Agency (ANVISA); Standard Methods of the Examination of Water and Wastewater (APHA); the Merck manual and the modified method ISO/TS 22964; Pitt; Hocking in Fungi and food; mycotoxins handbooks of FAO/WHO Expert Committee on Food Additives (JECFA). The analyzes were carried out in the laboratories of the State Center for Food Research at PESAGRO-RJ. Ten brands were collected from 8 different lots. Classification with a bimestrial difference at different market stablemen’s, with intention to make an average per brands (total of 80 samples). It was observed the following minimum and maximum variations in the counts for the proposed incubation schemes: DRBC (2.00 to 4.86); DG18 (1.88 to 4.40); YPD (2.00 to 4.90); DCPA (2.0 to 3.90). Trials of the natural incidence of mycotoxins demonstrated detectable levels of AFM1 (0.023 - 0.050 ug kg⁻¹) in the samples evaluated (Table 1). All brands of milks supplements analyzed by AFM1 incidence agree Brazilian legislation standards, but some trials exceed international legislation. The fungi count exceeded the stipulated by APHA and FDA/ FAO. Such contamination confers potential risk to consumers. Table 1. Fungi count (log10 cfu g⁻¹) in the Dichloran Rose Bengal Chloramphenicol agar (DRBC) and aflatoxin M1 concentration (ug kg⁻¹), in milk food supplement samples

Brand	DRBC	AFM1
X	4.06^a	0.0415^A
Y	3.41^b	0.0365^A
Z	3.30^b	0.0345^A
W	3.29^b	0.0353^A
A	3.06^b	0.0425^A
B	4.41^a	0.0505^A
C	4.30^a	0.0245^A
D	3.69^b	0.0233^A
E	3.16^b	0.0345^A
F	3.41^b	0.0365^A
Mean	3.60	0.3596

^a,b,AMeans with the same letter in column are equivalent in accordance with Duncan test (P ≤ 0.005).

Key Words: Aspergillus flavus, mycotoxin production, aflatoxin M₁