Abstract #M250

# M250
Effects of combinations of prilled fatty acids with or without potassium carbonate on fermentation and biohydrogenation intermediates in continuous culture fermenters.
L. E. Koch*1, B. M. Koch1, S. M. Hussein1, V. R. Trutwin1, T. C. Jenkins1, C. Soderholm2, J. Linn2, J. Albrecht2, G. J. Lascano1, 1Clemson University, Clemson, SC, 2Milk Specialties Global, Eden Prairie, MN.

The addition of buffers such as K2CO3 have been investigated in how they alter ruminal fermentation and reduce accumulation of milk fat inhibitors (MFI). Thus, we hypothesized that prilled saturated free fatty acids (FFA; C16:0 and C18:0) combined with K2CO3 would provide a slower, more prolonged release of K2CO3 than feeding it alone in reducing production of MFI. Four treatments were randomly assigned to 8 continuous culture fermenters for 2 periods of 10 d. Treatments included 4 combinations of FFA (Supplement A) and K2CO3 coated with prilled fatty acids (1:1; Supplement B) representing 1.25:0 (1), 0.83:0.83 (2), 0.42:1.66 (3), and 0:2.5% DM (4) of supplement A to B ratios. All treatments provided 1.25% DM of FA with K2CO3 increasing gradually from 0 to 1.25% DM. Data were analyzed using the MIXED procedure of SAS as a randomized complete block design with blocks of period and fermenters; where linear, quadratic and preplanned polynomial contrasts where evaluated. Addition of K2CO3 altered pH and biohydrogenation (Table 1). There was a quadratic increase in total VFA (P < 0.01) but butyrate was reduced linearly (P < 0.05), while valerate and isoacids increased quadratically with increasing K2CO3. Adding K2CO3 tended to increase the outflow of C18:1 (P = 0.09), C18:2 (P = 0.06) and reduce C18:3 (P = 0.06) linearly. Biohydrogenation intermediates, trans-10 and trans-12 18:1, were quadratically reduced with K2CO3. These results indicate that combinations of prilled fatty acids and K carbonate increase pH and reduce production of biohydrogenation intermediates linked to milk fat depression exhibiting a quadratic response. Table 1. Biohydrogenation and pH profile of fermenters fed 4 combinations of supplements A and B
ItemTRTSEContrasts, P-value
pH, h to<0.01
pH, h <<0.01
min pH5.575.635.695.590.100.690.19
max pH6.836.846.956.850.170.650.49

Key Words: lipid, biohydrogenation, continuous culture