The objective of this study was to compare rumen degradation kinetics and intestinal digestibility of wheat and corn dry distillers grains with solubles (DDGS) or without solubles (DDG). Three nonlactating Jersey cows with an average body weight of 436 ± 18 kg fitted with a rumen and T-type duodenal cannulas were used in the experiment. Six DDG products (3 from wheat, DDGSw1, DDGSw2, DDGSw3 and 3 from corn, DDGSc1, DDGSc2 and DDGc4) were collected from 4 ethanol plants. Feed samples were incubated in the rumen of the cows for 0, 2, 4, 8, 16, 24 and 48 h in 6 replications. The effective degradability of DM was higher (P < 0.05) for both wheat and corn DDGS products from ethanol plants 1 and 2 compared with DDGSw from plant 3 and DDGc from plant 4. The soluble fraction of CP ranged from 23.9% for DDGSw2 to 12.4% for DDGc4 with higher (P < 0.05) values for wheat DDGS than those from corn. Effective degradability of CP at different outflow rates (kp = 0.045, 0.06, and 0.08) for DDGSw1 and DDGSw2 were higher (P < 0.05) compared with those for corn DDGS. Correlation between color parameters (L – lightness, a- yellowness and b - redness) of the wheat DDGS and their CP effective degradability was high (r = 0.82 to 0.95). Further research is needed to evaluate the possibility of using these easily estimated color parameters as a proxy of the protein nutritional value of DDGS. The intestinal digestibility of rumen degradable DM, measured by the mobile bag technique, ranged from 52.0% for DDGSw3 to 38.3% for DDGc4 and was higher (P ≤ 0.05) for wheat DDGS compared with DDGSc1 and DDGc4. Intestinal digestibility of CP was not different (P > 0.05) among corn DDGS. Intestinal digestibility of CP from wheat DDGS was also highly variable (79.7% to 93.6%). Although nutrient composition of DDGS from different ethanol plants is highly variable, the protein degradability and digestibility values obtained in this experiment can be used in formulating rations for ruminant animals.