Abstract #M244

# M244
In vitro post-ruminal digestion of rumen bypass emulsions encapsulated by interfacial crosslinking using polyphenol oxidase from potato tuber peels.
F. Gadeyne*1, N. De Neve1, B. Vlaeminck1, P. Van der Meeren2, V. Fievez1, 1Laboratory for Animal Nutrition and Animal Product Quality, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, Ghent, Belgium, 2Particle and Interfacial Technology Group, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, Ghent, Belgium.

The protection of unsaturated fatty acids by emulsifying oil in a polyphenol oxidase (PPO) rich protein extract and cross-linking of interfacial protein upon 4-methylcatechol (4-MC) addition has been shown before as a promising technology to bypass rumen biohydrogenation (BH). An in vitro experiment was designed to assess the post-ruminal availability of linolenic acid (LNA) in rumen bypass emulsions. Therefore, emulsions (n = 3) were made with a protein extract of potato tuber peels, containing 20 mg triacylglycerols (TAG) of linseed oil per ml extract and a final 4-MC concentration of 0, 5, 10 or 20 mM, and were subjected to in vitro ruminal, abomasal and a time series of intestinal incubations. As TAG have to be hydrolyzed to nonesterified fatty acids (NEFA) before BH can occur, it was hypothesized protected LNA remained in the TAG fraction during ruminal incubation, but was released from its protective shell and concomitantly hydrolyzed to bio-available NEFA post-ruminally. Lipids were extracted from emulsions, after rumen or after the subsequent abomasal and intestinal incubation, separated in lipid fractions, methylated and analyzed using gas chromatography. Results were analyzed with the MIXED procedure of SAS. TAG LNA disappeared from the original emulsions after rumen incubation when no 4-MC was present (P < 0.001), but less in case of 5 (P = 0.006) or 10 (P = 0.024) mM 4-MC and no differences were observed anymore with 20 mM 4-MC (P = 0.879). All TAG LNA disappeared after abomasal and intestinal incubation compared with the original emulsions (P < 0.001), but LNA appeared partly as NEFA after abomasal and intestinal incubation when 4-MC was present (P < 0.004). Time series of intestinal incubations indicated, however, some level of overprotection, as LNA was least released in the NEFA fraction at the highest levels of rumen protection. In conclusion, dietary LNA in PPO-protected emulsions became bio-available for intestinal uptake, as a fraction of 0.223, 0.237, or 0.303 for emulsions with 5, 10 or 20 mM 4-MC, respectively, survived rumen BH and was released as NEFA after abomasal and intestinal incubations.

Key Words: polyphenol oxidase, biohydrogenation, rumen bypass