The objective of this study was to determine the cell wall (CW) composition along the corn stalk. Three phytomers of corn plants from 8 corn hybrids were used (2 replicates per hybrid). A center phytomer (C) corresponded to the phytomer of ear insertion. Upper (U) and lower (L) phytomers corresponded to 3 phytomers above and below, respectively, relative to phytomer C. Each phytomer was cut into 4 sections: top internode (T), middle internode (M), bottom internode (B), and node (N). For each replicate of each hybrid, sections within phytomers from 5 plants were composited, and the CW concentration and composition were determined. For CW composition, the concentrations of uronic acids (UA), arabinose (ARA), xylose (XYL), and glucose (GLU) were determined. Data were analyzed as for a randomized complete block design with repeated measures (subject = phytomer). The CW concentration did not change between phytomers (P < 0.34) but increased from B to T within the phytomer (30.3 to 35.1% DM, respectively; P < 0.01). Section N had the highest concentration of CW (37.2% DM). The UA concentration did not change between phytomers (P < 0.69) but decreased from B to T within the phytomer (4.2 to 3.3% CW, respectively; P < 0.01). Section N had the highest concentration of UA (5.7% CW). The concentration of ARA was greater in the U than in the L phytomers (3.0 vs. 2.1% CW; P < 0.01) and increased from B to T within the phytomer (1.8 to 2.2% CW, respectively; P < 0.01). Section N had the highest concentration of ARA (3.3% CW). The concentration of XYL was greater in the U than in the L phytomers (20.9 vs 18.9% CW; P < 0.01) and increased from B to T within the phytomer (18.7 to 19.8% CW, respectively; P < 0.01). Section N had the highest concentration of XYL (20.1% CW). The concentration of GLU did not change between phytomers (P < 0.12) but decreased from B to T within the phytomer (36.2 to 34.9% CW, respectively; P < 0.01). Section N had the lowest concentration of GLU (31.8% CW). Concentrations of UA, ARA, and XYL decrease and the concentration of GLU increases in more mature sections of the phytomer of the corn plant, showing changes in nutritional quality.