Abstract #147
Section: ADSA Production PhD Oral Competition (Graduate)
Session: ADSA Graduate Student (PhD) Production Oral Competition
Format: Oral
Day/Time: Monday 3:30 PM–3:45 PM
Location: 309
Session: ADSA Graduate Student (PhD) Production Oral Competition
Format: Oral
Day/Time: Monday 3:30 PM–3:45 PM
Location: 309
# 147
Feeding increasing amounts of ruminally-protected choline (RPC) increasingly reduced fatty liver of Holstein cows.
M. G. Zenobi*1, A. M. Lopez1, J. E. Zuniga1, M. B. Poindexter1, T. L. Scheffler1, S. R. Campagna2, B. A. Barton3, J. E. P. Santos1, C. R. Staples1, 1University of Florida, Gainesville, FL, 2University of Tennessee, Knoxville, TN, 3Balchem Corp, New Hampton, NY.
Key Words: choline, fatty liver
Feeding increasing amounts of ruminally-protected choline (RPC) increasingly reduced fatty liver of Holstein cows.
M. G. Zenobi*1, A. M. Lopez1, J. E. Zuniga1, M. B. Poindexter1, T. L. Scheffler1, S. R. Campagna2, B. A. Barton3, J. E. P. Santos1, C. R. Staples1, 1University of Florida, Gainesville, FL, 2University of Tennessee, Knoxville, TN, 3Balchem Corp, New Hampton, NY.
Objective was to determine the optimal feeding amount of choline to reduce the triacylglycerol (TAG) concentration of liver and to increase absorption of TAG from the small intestine of dairy cows. Pregnant, nonlactating multiparous Holstein cows (n = 77) were enrolled at 64 ± 10 d before calculated calving date. Dietary treatments were 0, 30, 60, 90, and 120 g/d of ReaShure (RPC; Balchem Corp., New Hampton, NY). Experimental periods were 14 d during which cows ate ad libitum (AL) amounts of feed for 5 d and were restricted (R) to consume 36% of their energy/protein requirement for pregnancy and maintenance for 9 d to simulate lactating cows in negative energy balance. Methionine intake was similar during the AL and R periods due to methionine supplementation. Liver was biopsied on d 5 and 14. A saturated fatty acid (454 g of Energy Booster Mag, Milk Specialties, Eden Prairie, MN) was fed after biopsy on d 14 and blood plasma measured for TAG for 19 h post feeding. Data were analyzed using Proc Mixed procedures of SAS. Birth weight of the calves and number of days before calving at the time of enrollment were used as covariates. Significance was declared at P < 0.05. Dry matter intake and energy balance decreased from 9.5 to 3.3 kg/d and from 3.6 to −8.2 Mcal NEm/d during the AL and feed R periods, respectively. Concentration of liver TAG was not affected by RPC during the period of AL feed intake (2.3% of liver DM) but decreased linearly with increasing intake of RPC from 17.5 to 11.1% during the period of feed R. With increasing intake of RPC, concentration of liver glycogen increased linearly during the period of AL feed intake and tended to increase quadratically during the period of R feed intake, being greater in liver of cows fed RPC compared with that of control cows. Mean plasma concentration of TAG (0, 5, 7, 9, 11, 13, 15, 17, and 19 h after fat consumption) increased quadratically with increasing intake of RPC (13.6, 17.4, 16.9, 15.9, and 15.7 mg/100 mL). Supplementing RPC from 0 to 120 g/d improved liver status and fat absorption of feed-restricted pregnant, nonlactating multiparous Holstein cows.
Key Words: choline, fatty liver
