Sugarcane is a high-energy yielding crop, and its silage can be used as an alternative feed for dairy cows in tropical regions. The objective of this study was to evaluate the effect of a bacterial additive (BAC),) and cellulase enzyme (ENZ) on chemical composition and fermentation of sugarcane silage. Inclusion or no inclusion of a BAC (Lactobacillus plantarum and Pediococcus pentosaceus, strains CH5796 and 2354, respectively; 2.5 × 10¹⁰ cfu/g) and 3 doses of ENZ, 0, 3 and 6% wt/wt (g cellulase/100g cellulose) added on sugarcane silage were evaluated after 30 d of fermentation in a completely randomized design with a factorial arrangement. Sugarcane was harvested at about 24.7% DM and 15.9 ^oBrix and ensilaged in 18 4-L capacity PVC mini-silos with 800kg/m³ density. At the opening, the silos presented silage suitable for consumption, considering color, smell, and visual absence of fungi and molds. Since titratable acidity is highly correlated with total acid levels, it was used as an indicator of lactic acid and responded to BAC and ENZ. Both additives improved anaerobic fermentation and stability after 24 h of sugarcane silages. BAC and ENZ significantly (P < 0.05) influenced DM, NDF, ADF, titratable acidity and aerobic stability. No interaction effect was observed. ENZ adding also affected hemicellulose content (Table 1). Table 1. Effect of a bacterial inoculant (Lactobacillus plantarum and Pediococcus pentosaceus) and cellulase adding on sugarcane silage composition¹

Item	BAC		P-value	ENZ			SEM	P-value
	C	BI		0%	3%	6%		Linear	Quadratic
DM	22.0	22.6	0.04	22.7	22.9	21.3	0.20	0.04	0.04
OM	96.0	95.9	0.19	96.0	96.1	95.8	0.01	0.19	0.19
NDF	58.3	55.6	0.02	61.0	55.9	54.0	0.86	0.02	0.02
ADF	32.9	29.8	<0.01	33.6	29.8	30.6	0.47	<0.01	<0.01
Hemicellulose	25.8	25.8	0.99	27.8	26.1	23.4	0.85	0.99	0.99
CP	3.0	3.0	0.61	3.0	2.9	3.0	0.04	0.61	0.61
pH	3.3	3.3	0.55	3.4	3.3	3.3	0.02	0.55	0.55
Titratable acidity (NaOH mL)	12.3	14.0	<0.01	11.9	13.5	14.1	0.34	<0.01	<0.01

¹C = Control; BI = bacterial inoculated sugarcane silage; ENZ = cellulase.