Taking into account that punicalagin is a very powerful anti-oxidant and very abundant in pomegranates and pomegranate juice, the present study evaluated the potential subacute toxic effects of oral punicalagin in male New Zealand White rabbits. A total of 24 male rabbits was used in the experiment. The rabbits were kept under standard laboratory conditions for 2 weeks before the experiment for adaptation. After adaptation, rabbits were divided into 4 groups of 6; positive controls (tap water), and 3 different doses of punicalagin in tap water (1, 2 and 10 mg/kg punicalagin). All treatments were administered by daily oral gavages for 9 weeks. Once a week, body weights and feed intakes of the rabbits were evaluated and the oral doses were adjusted accordingly. On d 63 of the experiment, blood from the ear artery was collected and used for hematological and biochemical parameters. After blood collection, rabbits were sacrificed and internal organ weights were taken. No significant differences were apparent in treated rabbits in any of the hematological (such as hemoglobin, hematocrit, red blood cells, mean corpuscular hemoglobin, mean corpuscular volume, mean corpuscular hemoglobin concentrations, total white blood cells, lymphocytes, monocytes, granulocytes, platelets, mean platelet volume) or biochemical (serum glucose, cholesterol, triglyceride, urea, creatine, alkaline phosphatase, aspartate aminotransferase, and alanine aminotransferase) parameters (P > 0.1). Body weight changes and feed intakes were similar among the treatments with no significant adverse effects throughout the study. Moreover, no alterations on organ weights were observed among the groups (P > 0.1). Thus, our subacute 63-d study suggested the lack of toxic effects of punicalagin even with 10 mg/kg daily intake in male New Zealand rabbits.