It has been reported that non-digestible saccharides such as difructose anhydride III (DFA III), fructooligosaccharides (FOS), and maltitol (MAL) enhanced the intestinal calcium absorption by acting on intestinal epithelium tight junctions. We have already shown that DFA III promoted apparent efficiency of absorption (AEA) of immunoglobulin G (IgG) and serum IgG concentration in newborn calves (Htun et al., J. Dairy Sci. 99:5701–5706), but effects of FOS and MAL on AEA and serum IgG concentration have not been investigated yet. This experiment was done to investigate the effects of FOS and MAL supplementation on AEA and serum IgG concentration in newborn calves. Eighteen newborn Holstein male calves were randomly assigned to receive no supplement (Control group, n = 6), or 18 g of FOS supplement (FOS group, n = 6), or 18 g of MAL supplement (MAL group, n = 6) in each feeding. All calves were fed 2 packages of colostrum replacer providing 120 g of IgG at 1 and 10 h after birth. After colostrum replacer feeding, 250 g/2 L of milk replacer was fed at 24 h, 36 h and then twice daily for 7 d after birth. Blood samplings were done before feeding at 0 h, 10 h, 24 h, 36 h, 4 d and 7 d of age for IgG analysis. One-way ANOVA followed by LSD multiple comparisons tests were used to compare individual parameters among the groups. Serum IgG concentration sharply increased from 0 h of age and reached a peak level at 24 h of age in all groups. Mean serum IgG concentration at 24 h of age in the MAL group (26.3 g/L) was higher than those in the control (20.0 g/L) and the FOS (19.1 g/L) groups (P < 0.05). Mean AEA at 24 h of age in the MAL group (42.3%) was higher than those in the control (31.4%) and the FOS (30.3%) groups (P < 0.05). In conclusion, MAL could improve both AEA and serum IgG concentration in newborn calves by feeding it with the colostrum replacer, but the effects of non-digestible saccharides on passive immune transfer in newborn calves differ with their types.