Because of the possible effects of bisphenol A (BPA) on human and other animals, there are several studies about the possible effects of BPA toxicity. Even so, the current literature lacks studies about the potential effects of BPA on rabbits. Therefore, the objectives of the present study were to document the effects of different doses of bisphenol A (BPA) on hematological and biochemical parameters, liver enzymes, weight gain and feed intake of male New Zealand White rabbits. Prior to the experiment male rabbits (n = 24) were acclimatized to laboratory conditions for 14 d. After the adaptation, rabbits were divided into 4 groups of 6; positive controls (Group 1, corn oil), and 3 different doses of BPA (10, 20 and 100 mg/kg BPA in corn oil) for 9 weeks. Body weights and feed intakes of the rabbits were evaluated weekly. At the end of the experiment blood samples from the ear artery were taken for the analyses of hematological and biochemical parameters. PROC GLM procedure was used for statistical evaluations. To compare the individual means of the groups, Dunnett post hoc analysis was performed. The results of the current study indicated no changes in weight gain and feed intake among the treatments. Similarly, the mean values of total white blood cells, lymphocytes, monocytes, granulocytes, platelets, mean platelet volume, mean corpuscular volume and mean corpuscular hemoglobin were within the physiological ranges for rabbits and not affected by BPA treatment at the end of the study. However, hemoglobin, red blood cells and mean corpuscular hemoglobin concentrations were reduced significantly due to 20 and 100 mg/kg BPA exposure (P < 0.05). Among the biochemical parameters, serum glucose, cholesterol, and triglyceride concentrations were not affected by BPA treatments. On the other hand, serum alkaline phosphatase, aspartate aminotransferase, alanine aminotransferase, urea, and creatine levels were significantly elevated in the 20 and 100 mg/kg BPA dose groups (P < 0.05). In conclusion, the current subacute study suggested a no-observed adverse effect level (NOAEL) of 10 mg BPA/kg in male New Zealand White rabbits.