Increasing milking frequency (MF) increases milk yield (MY) and farm profit. Early lactation increased milking frequency (IMF) involves milking cows 4 times daily (4×) for the first 21 d post-calving followed by twice-daily milking (2×) for the remainder of lactation. Increasing MF in early lactation increases production during and after the IMF period. Milking intervals (MI) describes the timing of daily milkings. Uneven 4× MI in early lactation may be easier to apply because employees can milk 4× cows at the beginning and end of 2× milking sessions. The objective of this experiment was to compare the MY effect of even and uneven 4× MI in early lactation IMF. Fourteen multiparous and 6 primiparous cows were milked using unilateral frequent milking with right udder halves milked 4× and left udder halves milked 2× for 20 d in early lactation. Ten (7 multiparous and 3 primiparous) cows were allocated evenly based on parity and assigned to either the even or uneven MI groups. The uneven and even MI groups were assigned to 9:3:9:3 h or 6:6:6:6 h interval respectively. Milk from each udder half was weighed and sampled for components on the final day of treatment and at 60, 120, 180, 240, and 300 DIM. On d 20, 4× udder halves produced 4.78 ± 1.02 kg and 7.31 ± 0.99 kg more milk than the 2× halves for the even and uneven MI groups. There was no significant difference between MI groups for udder half difference on d 20 (P = 0.07) or throughout lactation. The increased MY carry-over effect in 4× udder halves averaged 1.07 ± 0.55 kg/d for the even MI group and 0.63 ± 0.28 kg/d for the uneven group through 300 DIM. There was no significant MY difference between udder halves after cows were returned to 2× milking. IMF increases labor costs and uneven MI may optimize net income from increased MY minus elevated labor costs. This project was supported by Agriculture and Food Research Initiative Competitive Grant 2017-67015-26538 from the USDA National Institute of Food and Agriculture.