Abstract #540

# 540
Effects of 3-nitrooxypropanol on enteric methane emission and lactational performance of dairy cows.
A. Melgar*1, C. F. A. Lage1,2, K. Nedelkov1,3, S. E. Räisänen1, H. Stefenoni1, M. E. Young1, X. Chen1,4, J. Oh1, S. Duval5, M. Kindermann5, N. D. Walker5, A. N. Hristov1, 1The Pennsylvania State University, University Park, PA, 2Universidade Federal de Minas Gerais, Minas Gerais, Brazil, 3Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria, 4College of Pastoral Agriculture Science and Technology, Lanzhou University, China, 5DSM Nutritional Products, Kaiseraugst, Switzerland.

This study examined the effect of 3-nitrooxypropanol (3NOP), an investigational substance, on enteric methane (CH4) emission and lactational performance of Holstein dairy cows housed in a free-stall barn. Following a 3-wk covariate period, 40 multi- and primiparous cows averaging (±SD) 118 ± 28 d in milk (DIM), 43.4 ± 8 kg/d milk yield, and 594 ± 57 kg body weight (BW) were blocked based on covariate DIM, milk yield, and enteric CH4 emission and randomly assigned to one of 2 treatments: (1) control, no 3NOP, and (2) 3NOP applied at 60 mg/kg feed dry matter. 3NOP was incorporated in the total mixed ration and fed for 15 consecutive wks. Enteric gaseous emissions (CH4, carbon dioxide, and hydrogen) were measured using 3 GreenFeed units. Data were analyzed using PROC MIXED of SAS as repeated measures [ar(1) covariance structure] and with block and block × treatment as random effects. Dry matter intake (DMI, 25.6 kg/d; SEM = 0.38), cow BW (617 kg; SEM = 2.89), and BW change (458 g/d; SEM = 50.9) were not affected (P ≥ 0.45) by 3NOP. Compared with the control, 3NOP decreased (P < 0.001) CH4 daily emission by 26% (302 vs. 411 g/d; SEM = 6.16); decreased (P < 0.001) CH4 emission yield (11.9 vs. 16.4 g/kg DMI; SEM = 0.25), and emission intensity [8.2 vs. 11.5 g/kg energy corrected milk (ECM); SEM = 0.28]. Hydrogen emission increased (P < 0.001) by 3NOP from 0.44 g/cow/d (control) to 2.55 g/cow/d (SEM = 0.119). Carbon dioxide emission was not affected by treatment (average of 13.3 kg/cow/d; SEM = 1.16; P = 0.27). Treatment had no effect (P ≥ 0.45) on milk and ECM yields, averaging 38.4 kg (SEM = 0.63) and 37.2 kg/d, (SEM = 0.59), respectively. ECM feed efficiency was also not affected (P ≥ 0.94) by 3NOP. Milk protein and lactose concentration and yield were not affected (P ≥ 0.26) by treatment. 3NOP increased (P = 0.01) milk fat concentration (4.08 vs. 3.83%, respectively; SEM = 0.064) and tended to increase (P = 0.07) fat yield. In this experiment, 3NOP decreased enteric CH4 daily emission, yield, and intensity without affecting DMI and milk yield, but increased milk fat in lactating dairy cows.

Key Words: 3-nitrooxypropanol, enteric methane, dairy cattle