Abstract #M29
Section: ADSA Production PhD Poster Competition (Graduate)
Session: ADSA Production PhD Poster Competition (Graduate)
Format: Poster
Day/Time: Monday 7:30 AM–9:30 AM
Location: Exhibit Hall A
Session: ADSA Production PhD Poster Competition (Graduate)
Format: Poster
Day/Time: Monday 7:30 AM–9:30 AM
Location: Exhibit Hall A
# M29
Effects of feeding moderate- or high-starch close-up diet to cows on response of newborn calves to intravenous injection of glucagon-like peptide 1.
Y. Inabu*1, J. Haisan2, M. Oba2, T. Sugino1, 1Department of Bioresource Science, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan, 2Department of Agricultural Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada.
Key Words: heifer calf, glucagon-like peptide 1, prepartum diet
Effects of feeding moderate- or high-starch close-up diet to cows on response of newborn calves to intravenous injection of glucagon-like peptide 1.
Y. Inabu*1, J. Haisan2, M. Oba2, T. Sugino1, 1Department of Bioresource Science, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan, 2Department of Agricultural Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada.
The objective of this study was to evaluate the effects of feeding moderate- or high-starch close-up diet to close-up cows on response of newborn calves to intravenously (i.v.) injected glucagon-like peptide 1 (GLP-1). Holstein heifer calves (n = 37) born to cows fed a moderate- (M, 14% starch; n = 17) or high-starch (H, 26% starch; n = 20) diet during the last 28 d of gestation were assigned to one of 2 treatment groups, which were i.v. injected with saline as control (MC and HC, n = 9 and 10, respectively) or GLP-1 solution (MG and HG, n = 8 and 10, respectively) immediately after milk replacer (MR) feeding finished (within 5 s after MR feeding finished) at 2, 10, and 20 d after birth. Blood samples were collected at −10, 0, 10, 20, 30, 40, 50, 60, 90, and 120 min relative to treatment injection and plasma glucose, insulin, and GLP-1 concentrations were measured. Data were analyzed by ANOVA using fit model procedure of JMP® 14 pro (SAS Institute Inc., Cary, NC). Plasma GLP-1 concentration increased rapidly after GLP-1 injection and was higher for the calves injected with GLP-1 than those injected with saline (P < 0.01) at 2 (1.97 vs 1.02 ng/mL for G and C, respectively), 10 (2.10 vs 0.67 ng/mL for G and C, respectively) and 20 d after birth (1.79 vs 0.38 ng/mL for G and C, respectively), but no difference was observed between MG and HG at all sampling days. Both in M and H calves, the rise in postprandial plasma glucose concentration was suppressed (P < 0.01) by direct glucose-lowering action of i.v. injected GLP-1 at 10 (137 vs 150 mg/dL for G and C, respectively) and 20 d after birth (147 vs 158 mg/dL for G and C, respectively), and similar tendency was observed at 2 d after birth (133 vs 144 mg/dL for G and C, respectively; P = 0.09): this direct glucose-lowering action by GLP-1 was greater (P = 0.02) for H than for M calves at 20 d after birth (141 vs 152 mg/dL for HG and MG, respectively). These results indicate that feeding a high-starch diet to cows in close-up period enhances glucose-lowering action by GLP-1 after feeding depending on age of calves, which can affect glucose status in newborn calves.
Key Words: heifer calf, glucagon-like peptide 1, prepartum diet
