The objective of this study was to determine the effect of SNP in growth hormone receptor (GHR), IGF-I, and tumor necrosis factor α (TNFa) on hepatic gene expression. Multiparous Holstein cows (n = 40) were part of a separate study evaluating effects of diet on postpartum parameters. Cows were fed a common prepartum ration and assigned randomly at calving to one of 2 postpartum diets differing in fiber content. High fiber (35.3% NDF, 12.2% uNDF₂₄₀, 23.2% peNDF; n = 27) and low fiber (32.8% NDF, 9.5% uNDF₂₄₀, 21.6% peNDF; n = 29) treatment diets were formulated for equivalent metabolizable protein and starch. DNA from blood of each cow was genotyped for all SNPs by PCR-restriction fragment length (PCR-RFLP) methods. Liver biopsies (n = 40) were obtained on d 7 ± 1.1 (mean ± SD) and 14 ± 1.0 postpartum, liver tissue was immediately frozen in liquid nitrogen. Liver samples were analyzed via RT-qPCR to evaluate mRNA expression of GHR, IGF-I, TNFa, and tumor necrosis factor receptor super family 1a (TNFRSF1). Data were analyzed by repeated measures ANOVA with the random effect of cow within treatment and fixed effects of dietary treatment, genotype for all genes, time, parity group, and all interactions. There were no main effect differences (P > 0.15) of dietary treatment on hepatic gene expression of IGF-I, GHR, TNFa, or TNFRSF1. Cows with GHR SNP AB tended (P < 0.15) to have higher hepatic IGF-I gene expression compared with cows with GHR SNP AA or BB, while cows with TNFa SNP AB tended (P < 0.15) to have lower hepatic IGF-I gene expression compared with those with TNFa SNP BB. Hepatic gene expression was also influenced by GHR SNP such that genotype AB cows tended (P < 0.15) to have higher hepatic GHR gene expression than cows with GHR SNP AA or BB. There were no effects (P > 0.15) of genotype on hepatic TNFa or TNFRSF1 gene expression; however both differed (P < 0.01, P = 0.12 respectively) over time, with higher expression at d 14 compared with d 7. Genotype of GHR and TNFa, but not IGF-I, influenced hepatic gene expression in postpartum dairy cattle.