The objective of this study was to evaluate the effect of source of trace minerals and corn silage on lactation performance and total-tract digestibility (TTD) of nutrients of Holstein cows. Fourteen cows averaging 82 (SE = 3) days in milk were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments with 28-d periods. The diets consisted (dry basis) of 55% conventional (CON) or brown midrib-3 (BM3) corn silage, 2% straw, and 43% grain mix with either sulfate (STM) or hydroxy (HTM; IntelliBond, Micronutrients USA) source of copper, zinc, and manganese trace minerals. The targeted levels of copper, zinc, and manganese were 194, 1657, and 687 mg/d, respectively. The dietary treatments were: 1) CON-STM, 2) CON-HTM, 3) BM3-STM, and 4) BM3-HTM. Cows were housed in tie stalls, fed TMR 1×/d, and milked 3×/d. Dry matter intake (DMI) and milk yield were measured daily from d 18 to 28. Milk composition was measured on d 25 and 26 and TTD was determined on d 21 to 23. Behavior was observed on d 23 to 25. Data were summarized by period and analyzed as a replicated Latin square design with fixed model effects for corn silage, trace mineral, corn silage and trace mineral interaction, period within replicate, and replicate using the MIXED procedure of SAS (version 9.4). Cow within replicate was a random effect. Cows fed the BM3 diets had greater (P ≤ 0.05) DMI (28.1 vs. 27.5 kg/d, SE = 0.5), milk yield (47.0 vs. 44.7 kg/d, SE = 1.1), and TTD of dry matter (72.8 vs. 71.1% of DM, SE = 0.6) and organic matter (OM; 74.1 vs. 72.3% of DM, SE = 0.5) and ruminated less (496 vs. 524 min/d, SE = 16, P = 0.01) than cows fed the CON diets. Cows fed the HTM diets had greater (P = 0.01) DMI (28.1 vs. 27.5 kg/d, SE = 0.5) and a tendency (P = 0.10) for higher TTD of neutral detergent fiber (aNDFom; (56.8 vs. 54.9% of DM, SE = 0.9)) than cows fed the STM diets. Corn silage with higher NDF digestibility affected DMI, milk yield, and TTD of DM and OM. Source of trace minerals can influence DMI and TTD of aNDFom, and should be taken into consideration when formulating diets for high producing dairy cows.