The dry period, an important resting period between lactations, is used in many dairies for long-term antibiotic treatment of intra-mammary infections and protection against new infections. However, there are concerns regarding the use of antibiotics due to the risk of developing antimicrobial resistance bacteria. Acoustic pulse therapy (APT) device developed to produce low power, deep penetration acoustic pulses distributed over a large treatment area was specifically designed for treating dairy cows. Previous experience with APT on cows with clinical and subclinical mastitis has shown bacterial elimination, increased milk yield and lower SCC. The aim of this study was to evaluate the effect of APT on cows during the dry period as an alternative to current dry cow therapies. Fifty cows, 21 APT and 29 control at 5 repetitions were treated during dry-off, 4–5 weeks before parturition (400 pulses/quarter for 3.5 min, frequency ~1.9Hz, energy density 0.041 mJ/mm²). All cows were intramammary treated by antibiotics. Cows were paired according to lactation, daily milk yield, DIM, SCC, history of infection, bacteriology and number of infected quarters. In each pair, one cow was assigned to APT and the second to control. Due to management, 1 repetition was not APT treated and all these cows were signed to control. When comparing the first 100 d following the dry-period to the first 100 d in the previous lactation period, an overall increase of 10% in milk yield was observed in the APT treated group compared with 1% in the control group (χ²). The differences were more notable in the 3rd lactation (11.6%), and the 4th lactation (13.7%). Average SCC in the first 100d was significantly lower in APT compared with control (101,000 cells/mL vs. 427,000 cells/mL). Previously we reported that APT was found more effective than antibiotics in treating clinical mastitis or no-intervention in sub-clinical mastitis in dairy cows. The current report suggests that APT treatment during the dry period can result in increasing angiogenesis which stimulates growth of new blood vessels that supply and oxygenate the treated area, support faster healing of the infected tissue and stimulates secretory cells and lobule.