Abstract #494

# 494
Increased high-moisture alfalfa hay preservation, in vitro ruminal degradability, fermentability, and decreased proteolysis with the novel use of paper mill byproducts.
D. C. Reyes*1, S. L. Annis2, A. Y. Leon-Tinoco1, S. A. Rivera1, H. M. Dubuc1, L. B. Perkins3, J. J. Perry3, R. J. Kersbergen4, C. Wu5, C. W. Knight4, M. S. Castillo6, J. J. Romero1, 1Animal and Veterinary Sciences, School of Food and Agriculture, University of Maine, Orono, ME, 2School of Biology and Ecology, University of Maine, Orono, ME, 3Food Science and Human Nutrition, School of Food and Agriculture, University of Maine, Orono, ME, 4University of Maine Cooperative Extension, Orono, ME, 5Department of Animal and Food Sciences, University of Delaware, Newark, DE, 6Department of Crop and Soil Science, North Carolina State University, Raleigh, NC.

We evaluated the effect of 2 previously optimized technical lignins, sodium and magnesium lignosulfonate (NaL and MgL, respectively), on the preservation and nutritive value of high-moisture alfalfa hay. Lignins and propionic acid (PRP; positive control) were added to 36 fresh g of sterile alfalfa hay (69.3 ± 0.6% DM), inoculated with a mixture of previously isolated spoilage fungi (5.6 log cfu/fresh g), and aerobically incubated for 15 d (25°C). Treatments were the factorial combination of 3 preservatives (NaL, MgL, and PRP) and 4 concentrations (0, 0.5, 1, and 3% wt/wt fresh basis). Data were analyzed as a randomized complete block design replicated 5 times and differences were declared at P ≤ 0.05. At d 0, the untreated hay had 47.8 ± 1.2, 11.1 ± 0.59, 66.2 ± 1.5, 30 ± 1.2 of NDF, sugars, and 24 h in vitro ruminal DM (DMD, % of DM) and NDF digestibility (NDFD, % of NDF), respectively. At d 15, relative to untreated (14.9 ± 0.77%), DM losses were lessened by doses as low as 1% for NaL (3.39) and 0.5% for PRP (0.81). This was explained by a reduced mold count for both NaL at 3% (3.92 ± 0.55 log cfu/fresh g) and PRP as low as 0.5% (3.94) vs. untreated (7.76). Consequently, sugars were best preserved by NaL at 3% (10.1 ± 0.283% DM) and PRP as low as 0.5% (10.5) vs. untreated (7.99), while keeping NDF values lower in NaL (45.9 ± 0.66% DM) and PRP-treated (45.1) hays at the same doses, respectively, vs. untreated (49.7 ± 0.66% DM). Hay DMD was increased by doses as low as 3% for NaL (67.5 ± 0.77%), 1% MgL (67.0), and 0.5% PRP (68.5) vs. untreated (61.8). In the case of NDFD, 0.5% for MgL and PRP (30.5 and 30.1 ± 1.09% DM, respectively) and 1% for NaL (30.7) were the lowest effective doses vs. untreated (23.3). At 3%, NaL decreased ruminal NH3-N (49.6 vs. 58.2 ± 1.50 mg/dL) and increased total volatile fatty acids (111.9 vs. 86.7 ± 1.3 mM) to the greatest extent vs. untreated, respectively. NaL was the best lignin-based preservative, but its effects were lesser than PRP. MgL stimulated digestibility independently of nutrient preservation.

Key Words: hay preservation, technical lignins, ruminal digestibility