Abstract #W70
Section: Physiology and Endocrinology (posters)
Session: Physiology and Endocrinology 2
Format: Poster
Day/Time: Wednesday 7:30 AM–9:30 AM
Location: Exhibit Hall A
Session: Physiology and Endocrinology 2
Format: Poster
Day/Time: Wednesday 7:30 AM–9:30 AM
Location: Exhibit Hall A
# W70
Pegbovigrastim treatment alters gene expression profiles of leukocytes in Simmental and Holstein cows after calving.
V. Lopreiato1, A. Minuti2,3, D. Britti1, F. Trimboli1, F. Piccioli Cappelli2, J. J. Loor4, E. Trevisi*2,3, 1Interdepartmental Services Centre of Veterinary for Human and Animal Health, Department of Health Science, Magna Græcia University, Catanzaro, Italy, 2Department of Animal Sciences, Food and Nutrition (DIANA), Università Cattolica del Sacro Cuore, Piacenza, Italy, 3Proteomics and Nutrigenomics Research Center (PRONUTRIGEN), Università Cattolica del Sacro Cuore, Piacenza, Italy, 4Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana, IL.
Key Words: gene expression, pegbovigrastim, leukocyte
Pegbovigrastim treatment alters gene expression profiles of leukocytes in Simmental and Holstein cows after calving.
V. Lopreiato1, A. Minuti2,3, D. Britti1, F. Trimboli1, F. Piccioli Cappelli2, J. J. Loor4, E. Trevisi*2,3, 1Interdepartmental Services Centre of Veterinary for Human and Animal Health, Department of Health Science, Magna Græcia University, Catanzaro, Italy, 2Department of Animal Sciences, Food and Nutrition (DIANA), Università Cattolica del Sacro Cuore, Piacenza, Italy, 3Proteomics and Nutrigenomics Research Center (PRONUTRIGEN), Università Cattolica del Sacro Cuore, Piacenza, Italy, 4Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana, IL.
Pegbovigrastim is a commercial long-acting analog of bovine granulocyte colony-stimulating factor. We hypothesized that pegbovigrastim (Imrestor; Elanco) administered at approximately 7 d before expected calving and within 24 h after calving affects expression profiles of genes involved in leukocyte function. Cows were randomly allocated into 1 of 2 groups: control [CTR; 13 Holstein (7 primiparous and 6 multiparous) and 13 Simmental (6 primiparous and 7 multiparous)] and pegbovigrastim [PEG; 13 Holstein (6 primiparous and 7 multiparous) and 13 Simmental (6 primiparous and 7 multiparous)]. Blood was collected on d 3 after calving in PAXgene tubes (Preanalytix) to measure mRNA expression of 36 genes. The final data were normalized using the geometric mean of 3 internal control genes: ACTB, SDHA, and YWHAZ. Data were subjected to ANOVA and analyzed with PROC MIXED of SAS. Treatment, breed, parity, and their interaction were the fixed effects. Compared with CTR, PEG cows had greater expression (P < 0.01) of genes involved in recognition and immune modulation (CD14, CD16, MYD88, TLR2, and TLR4), cell adhesion (ITGB2, ITGAL, TLN1, SELL, SELPLG, and CD44), antimicrobial activity (MMP9, LTF, LYZ, and LCN2), and inflammation (CASP1, TNFRSF1A, IL1B, IL1R, IL18, IRAK1, NRLP3, and S100A8). Pegbovigrastim also led to lower expression of RPL13A, ALOX15, IL8, TLN2, and TNF (P < 0.05). Expression of IDO1, RPL13A, ALOX5, CD44, CX3CR1, ITGB2, and TNF was greater in Simmental compared with Holstein (P ≤ 0.05), whereas expression of TLR2was lower (P < 0.05). Compared with multiparous cows, primiparous had greater expression of IL1B, IL18, MYD88, SELL, and TLR2 (P < 0.05), and lower expression of MMP9 (P < 0.05). Overall, pegbovigrastim have elicited an improvement of migration, adhesion, and antimicrobial ability, and an enhanced inflammatory response at 3 d after calving, that in turn might be beneficial for mounting a better response after the calving event and the homeorhetic adaptation to lactation. These preliminary results also unravel a different molecular response at innate and adaptive immunity level from different cattle breeds under the dairy management.
Key Words: gene expression, pegbovigrastim, leukocyte
