Abstract #W28

# W28
Effect of ensiling time and temperature on the fermentation and nutritive value of a corn hybrid containing an α-amylase enzyme trait (Enogen Feed Corn).
E. Hellings1, K. Moyer*1, É. da Silva1, C. Novinski3,1, Y. Li2,1, W. Sá4,1, R. Mester1, L. Kung Jr.1, 1University of Delaware, Newark, DE, 2Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, China, 3Federal University of Paraná, Curitiba, Paraná, Brazil, 4Federal University of Paraíba, João Pessoa, Paraíba, Brazil.

We evaluated the effect of time and storage temperature on the nutritive value of a corn hybrid containing an α-amylase enzyme trait (E; Syngenta Seeds, LLC, Minnetonka, MN) compared with its isogenic line (C). Whole-plant corn (38–39% DM) was packed in 7.5 L laboratory silos (208 kg of DM/m3) and ensiled at 22°C (low temperature) or at 40°C for 0 to 28 d, then at 32°C (high temperature) before opening after 28, 123 and 242 d of ensiling. Data were analyzed using the Fit Model in JMP (SAS Institute Inc., Cary, NC) with the main effects of hybrid, day, temperature, and their interactions. Concentrations of starch were not affected by length of storage, temperature, or hybrid. Water-soluble carbohydrates (WSC) were higher in E than C stored at the higher vs. lower temperature (P < 0.005), but were unaffected by storage length. Lactic acid was lower in E than C (P < 0.05), but pH remained the same. Acetic acid was not affected by temperature or hybrid but increased at 123 d (P < 0.005). Washout of starch from in situ bags at 0 h from fresh plants was 25.55% for E compared with 22.55% for C. There was no ensiling day (28 to 242 d) effect on 0 h loss of starch, but E stored at a higher temperature had more (P < 0.05) loss (55.67%) than C (32.24%). Seven h in situ starch digestion (STRD) of fresh plants was 54.39% for C compared with 73.15% for E. STRD was affected by all main effects. For hybrids, E had higher STRD than C (85.92 vs. 83.96%, P < 0.05). STRD increased with ensiling time (81.54% at 28 d, 85.39% at 123 d, and 87.88% at 242 d, P < 0.0001). Corn stored at a higher temperature had greater STRD than corn stored at a lower temperature (88.13 vs. 81.75%, P < 0.0001). Type of hybrid did not affect the aerobic stability (h before a 2°C rise after exposure to air at 21°C) of silages. Greater WSC and STRD in E stored at elevated temperatures suggests expression of α-amylase activity during storage. The corn hybrid containing an α-amylase enzyme trait shows promise in providing increased STRD while maintaining a favorable fermentation during ensiling.

Key Words: corn silage, α-amylase, starch digestibility