SexedULTRA is a method of sperm sorting and processing that provides a benign environment to improve the quality of ejaculates and retain sperm cell integrity. The aim of this study was to evaluate if the in vitro characteristics of below standard quality ejaculates can be improved by SexedULTRA processing. Two low quality ejaculates from 6 bulls were split, processed, and cryopreserved as non-sorted (NS), SexedULTRA bulk-sorted (SBS) and sex-sorted (SSS). Percent head and tail morphologies were evaluated visually after thawing (0 h). Sperm motility was estimated visually (MOT) and classified into total (TOT) and progressive (PROG) using a computer assisted sperm analyzer after thawing and post-incubation (3 h at 36°C). Percent viability (VIA) and acrosome integrity (PIA) were assessed using flow cytometry after thawing and post-incubation. Sperm DNA fragmentation index (DFI) was estimated using the Halomax Kit immediately after thawing and after 6, 24, 48 and 72 h of incubation at 36°C. In vitro fertilization (IVF) was performed as a measure of sperm competence using NS and SSS straws from 4 of the ejaculates. Two hundred oocytes per treatment group (ejaculate × treatment) were included in the comparison for embryo production. Data were analyzed by a mixed model in conjunction with a Tukey contrast with the fixed effect of treatment and time, and random effect of ejaculate. SSS and SBS sperm head and tail abnormal morphologies were significantly lower (P < 0.05) when compared with NS sperm. SexedULTRA had lower (P < 0.05) DFI than NS sperm at every time of incubation. SSS and SBS sperm also presented numerically higher MOT, TOT, PROG, VIA and PIA than NS sperm, but differences were not significant. During incubation of NS sperm, a strong time by treatment effect (P < 0.05) was seen for MOT, TOT and PROG. This effect was not present for SSS or SBS sperm. The percent of embryos produced was significantly higher (P < 0.05) for SexedULTRA sperm. The results show that SexedULTRA processing can remove morphological issues, improve sperm quality and increase embryo production of low-quality ejaculates, allowing for samples that would otherwise be discarded, to be processed as a bulk- or sex-sorted product.