Deworming is a common practice at dairy farms. Limited data are available to indicate the influence of deworming on behavior of dairy cows. The objective of current study was to assess the influence of deworming on feeding and lying behavior of Holstein cows. Twenty Holstein cows of second parity having 25.8 ± 4.8 kg/d milk and 215 ± 21 DIM (mean ± sd) were simultaneously enrolled from dairy herd at University of Veterinary and Animal Sciences, Lahore Pakistan. The data loggers (Nedap CowControl: Nedap N.V., The Netherlands) were attached to neck and leg of enrolled animals to record feeding and lying behavior, respectively. The observation period lasted for 4 d and categorized as day before (d −1), day of (d 0), d 1, and d 2 of deworming. The behavioral responses were analyzed using Proc mixed procedure of SAS with planned contrasts where d −1 was taken as control. Total feeding bouts tended to increase on d 1 after deworming (13.1 vs. 13.8 number/d; d −1 vs. d 1; SE = 0.46; P = 0.150) and became similar on d 2. Interestingly, total feeding time was similar for d −1, d 0, and d 1 of deworming but it was significantly lower for d2 (487.9 vs. 442.3 min/d; d −1 vs. d 2; SE = 18.2; P = 0.025). Total lying bouts tended to increase on and after deworming (8.2 vs. 8.6, 8.5, and 8.6 number/d; d −1 vs. d 0, d 1, and d 2; SE = 0.26; P = 0.090, d −1 vs. d 2). However, total lying time was similar on and after one day of deworming but it significantly increased on d 2 of deworming (620.5 vs. 678.6 min/d; d −1 vs. d 2; SE = 0.46; P = 0.019). These results indicated that deworming practice did not significantly influence feeding and lying behavior on and one day after deworming. The unpredictable behavioral response on d 2 of deworming could be confounded due to environment or farm management.