The objective was to determine whether Holstein calves exposed to choline biomolecules in utero and through colostrum would attenuate LPS-induced inflammatory responses. Male calves (n = 38) received 1 of 4 treatments in a 2 × 2 factorial arrangement; namely, pregnant dams fed 0 or 12.9 g/d of ruminally protected choline ions (RPC; ReaShure; Balchem Corp.) beginning 21 d before expected calving and colostrum (3.8 L within 2 h of birth) produced by dams supplemented with 0 or 12.9 g/d of RPC ions. At 21.5 ± 2.3 d of age, calves were given i.v. lipopolysaccharide (LPS; 0.05 µg/kg of BW, E. coli serotype O111:B4). Unless otherwise stated, measurements were taken at 0, 1, 2, 3, 4, 6, 8, 10, 12, 24, 30, 36, 48, 60, and 72 h relative to LPS injection. Mean amount of IgG fed (430 g) and mean intake of DM (775 g/d) before LPS did not differ due to treatment, but mean intake of DM was greater (1,074 vs. 976 g/d, P < 0.05) after the LPS challenge (0 to 8 d) by calves born from dams fed RPC compared with calves born from control dams. Compared with calves born from control dams, calves born from RPC-fed dams had lower mean rectal temperature (39.0 vs. 39.2°C; P = 0.03) and mean respiration rate (35.6 vs. 39.3 breaths/min; P = 0.05). Plasma concentrations of BHB and NEFA were lower whereas those of glucose were greater within the first 6 to 8 h after LPS (choline in utero by time; P < 0.01). Mean plasma concentrations of total Ca tended to be greater (10.1 vs. 9.6 mg/dL; P = 0.08) but those of zinc tended to be lower (0.79 vs. 0.91 mg/L; P = 0.07) for calves born to dams fed RPC compared with control calves. Mean plasma concentrations of cortisol (0 to 24 h; 16.5 vs. 19.5 ng/mL; P = 0.03) and tumor necrosis factor-α (0 to 6 h; 1,308 vs. 1,846 pg/mL; P = 0.04) were lower and concentrations of serum amyloid A tended to be lower (choline in utero by time; P = 0.09) at 30 and 36 h after LPS for calves exposed to RPC in utero. Feeding colostrum from dams fed RPC prepartum had little effect on calf responses to LPS. Overall, inflammatory responses by male calves given LPS i.v. were modulated if born from dams fed RPC during late pregnancy.