Feed push-up improves the feed availability for dairy cows between feed deliveries and may increase intake and milk production. The study objective was to compare the effects of different feed push-up frequency of an automatic feed push-up system (Juno, Lely, Maasluis, Netherlands) on dairy cow behavior and milk production. Forty-eight cows (mean ± SD; 173 ± 101 DIM; 73.6 ± 23.3 lbs/d; 1.3 ± 0.5 parity) were divided into 2 groups, balanced by parity and DIM. A crossover design compared 2 treatments from April to June 2017: 2 feed push-ups/d (2FPU; 1000 and 1950 h) and 12 feed push-ups/d (12FPU; 0000, 0200, 0345, 0500, 0820, 1000, 1200, 1400, 1610, 1950, 2100, and 2200 h), for 3 wk/treatment, separated by 1 wk washout. Rumination time and eating time were measured by a behavior monitoring eartag; SmartBow (SmartBow GmbH, Jutogasse, Austria) and SensOor (Agis Harmelen, the Netherlands) respectively. Rest time was recorded by a behavior monitoring leg tag (Afi Act II, Afimilk, Afikim, Israel). An in-line milk assessment tool (AfiLab, Afimilk, Afikim, Israel) measured milk yield, milk fat percentage, and protein percentage. A mixed linear model (SAS 9.4) was used to determine the relationship of feed push-up frequency with behavior and milk production variables. Date was specified as a repeated measure, and cow as subject, using a compound-symmetry structure. The model contained the fixed effects of DIM, parity (1 or 2), treatment, sequence of treatment, THI, and all 2-way interactions (fixed effects remained regardless of significance; manual backward elimination removed non-significant 2-way interactions). Daily resting time was greater for 2FPU than 12FPU (640 ± 12 vs 615 ± 12 min/d; P < 0.001) and rumination tended to increase for 2FPU (366 ± 9 vs 359 ± 9 min/d; P = 0.06). Daily eating time and energy corrected milk were not different between treatments (P > 0.10). Feed push-up frequency influenced cattle behavior; however did not have a significant effect on feeding or production. Further research with feed push up frequency should consider intermediary frequencies and other variables as intake rate and feeding bouts.