Abstract #485

# 485
Lactational performance of dairy cows in response to supplementing N-acetyl-L-methionine as a source of rumen-protected methionine.
F. X. Amaro*1, K. G. Arriola1, Y. Jiang1, D. Kim1, A. P. Cervantes1, V. P. Silva1, M. C. N. Agarussi1, J. T. Silva1, A. T. Adesogan1, L. F. Ferraretto1, C. R. Staples1, J.-S. Eun2,3, J. S. Park3, J. O. Moon3, D. Vyas1, 1Department of Animal Sciences, University of Florida, Gainesville, FL, 2Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan, UT, 3Biotechnology Research Institute, CJ CheilJedang, Suwon, South Korea.

Adequate supply of essential and limiting amino acids such as methionine is critical for maintaining milk production by high-producing dairy cows. The objective was to assess the effects of supplementing incremental amounts of N-acetyl-l-methionine (NALM), a source of rumen-protected methionine, on DMI, milk production, and dairy efficiency in lactating dairy cows. Sixty multiparous Holstein dairy cows in early lactation (27 ± 4.3 d-in-milk) were fed for 16 wk in a randomized complete block design. Animals were grouped based on actual milk yield, and calving date. Dietary treatments included (1) control (no NALM); (2) 15 g/d NALM; (3) 30 g/d NALM; and (4) 45 g/d NALM. Diets were formulated to meet or exceed nutritional requirements of lactating dairy cows producing at least 42 kg/d of milk and to under supply metabolizable methionine (control) or supply increasing amounts with increasing NALM supplementation. Data were analyzed using the GLIMMIX procedure of SAS, using covariate in the model for all variables tested. Linear and quadratic effects with NALM were tested. Supplementation of NALM did not affect DMI (P = 0.37) regardless of the dose, whereas milk yield tended to be greater (P = 0.07) with intermediate levels of NALM (15 and 30 g/d) compared with the control. Milk true protein concentration was not influenced by supplementing NALM at 15 and 30 g/d, but these inclusion levels increased milk protein yield relative to the control (P = 0.04) possibly due to the increase in milk yield. Dairy efficiency (milk yield/DMI) was greatest at 30 g/d NALM (P = 0.03) and was unaffected by supplementing NALM at 15 or 45 g/d compared with the control. Supplementation of NALM at 30 g/d resulted in greatest milk yield, milk protein yield, and dairy efficiency when fed to early to mid-lactating dairy cows. However, the greatest amount of NALM supplementation (45 g/d) led to no beneficial effects on lactational performance. Overall results in the current study suggest that NALM supplementation can improve productive performance of dairy cows when added at 30 g/d.

Key Words: dairy efficiency, milk production, N-acetyl-l-methionine