Abstract #T83
Section: Dairy Foods (posters)
Session: Dairy Foods V: Microbiology
Format: Poster
Day/Time: Tuesday 7:30 AM–9:30 AM
Location: Exhibit Hall A
Session: Dairy Foods V: Microbiology
Format: Poster
Day/Time: Tuesday 7:30 AM–9:30 AM
Location: Exhibit Hall A
# T83
Raw milk microbiome of naturalized Brazilian breeds Curraleiro Pé-Duro and Pantaneiro.
Nayana R. Soares1, Marília C. Sola2, Clarice Gebara*1, Giovana V. Barancelli3, Ozana F. Zaccaroni4, Maria Clorinda S. Fioravanti4, Edmar S. Nicolau1, Cíntia S. Minafra-Rezende1, 1Food Research Center, School of Veterinary Medicine and Animal Science, Federal University of Goiás, Goiânia, Goiás, Brazil, 2Unified Higher Education Institute, Objetivo Faculty, Goiânia, Goiás, Brazil, 3Department of Agro-Industry, Food and Nutrition, “Luiz de Queiroz” College of Agriculture, University of São Paulo, Piracicaba, São Paulo, Brazil, 4School of Veterinary Medicine and Animal Science, Federal University of Goiás, Goiânia, Goiás, Brazil.
Key Words: milk microbiota, Bacillus sp., molecular analysis
Raw milk microbiome of naturalized Brazilian breeds Curraleiro Pé-Duro and Pantaneiro.
Nayana R. Soares1, Marília C. Sola2, Clarice Gebara*1, Giovana V. Barancelli3, Ozana F. Zaccaroni4, Maria Clorinda S. Fioravanti4, Edmar S. Nicolau1, Cíntia S. Minafra-Rezende1, 1Food Research Center, School of Veterinary Medicine and Animal Science, Federal University of Goiás, Goiânia, Goiás, Brazil, 2Unified Higher Education Institute, Objetivo Faculty, Goiânia, Goiás, Brazil, 3Department of Agro-Industry, Food and Nutrition, “Luiz de Queiroz” College of Agriculture, University of São Paulo, Piracicaba, São Paulo, Brazil, 4School of Veterinary Medicine and Animal Science, Federal University of Goiás, Goiânia, Goiás, Brazil.
Raw milk microbiota is complex and dependent of animal-environment ecosystem. Brazilian cattle breeds Curraleiro Pé-Duro and Pantaneiro represent important local genetic resources, given their socioeconomic impact and potential use in animal husbandry. Although they are not consider as specialized dairy breeds, is relevant to know the microbiota of raw milk, as potential for local exploration.The aim of this study was to identify the microorganisms present on raw milk from naturalized Brazilian breeds Curraleiro Pé-Duro and Pantaneiro through microbiome. Raw milk samples were aseptically collected from Brazilian breeds Curraleiro (6 animals) and Pantaneiro (5 animals) and submitted to molecular analysis. However, the samples were previously cultured on BHI broth, which is a standard protocol for food research, since naturally occurring fastidious microorganisms are hardly recovered in conventional assay in raw milk. Microbial community was determined through sequencing of amplicons from the V3 and V4 hypervariable region of the 16S rRNA gene that was amplified by PCR and optimized by Illumina TruSeq platform. A total of 740,907 quality-checked sequences were formed (394,710 from Curraleiro and 346,197 from Pantaneiro). Main genera identified on raw milk were Bacillus, present on 90.9% of samples and representing 65.97% of identified sequences for Curraleiro and 67.75% for Pantaneiro. Other identified genera and its respective sequences for Curraleiro (C) and Pantaneiro (P) samples were Enterococcus (10.50% C, 19.63% P), Enterobacter (10.19% C, 7.29% P), Staphylococcus (8.07% C, 0.08% P), Streptococcus (1.25% C, 0.16% P), Pseudomonas (1.20% C, 0.11% P), Lactococcus (0.02% C, 0.33% P) and Salmonella (0.24% P). Despite raw milk microbiota complexity, including pathogens, our hypothesis is that exist a balance between these microorganisms, once these animals presents low mastitis incidence and produce milk with low SCC. Results also highlight relevance of microbiome for understanding milk bacterial ecosystem and its importance for animal health as well as for technological and safety characteristics of milk.
Key Words: milk microbiota, Bacillus sp., molecular analysis
