Abstract #M260

# M260
In vivo evaluation of a new rumen-protected methionine supplement.
Hector L. Diaz*1, Jacob Albrecht1, Charles Soderholm1, Jim Linn1, Jeffrey Firkins2, Paul Kononoff3, John K. Bernard4, 1Milk Specialties Global, Eden Prairie, MN, 2The Ohio State University, Columbus, OH, 3University of Nebraska, Lincoln, NE, 4University of Georgia, Tifton, GA.

The objective of this research was to assess a new rumen-protected Met supplement (EBMet) for rumen undegradability, intestinal and total-tract CP digestibility and blood plasma AA response. EBMet contains 34% DL Met coated with saturated FA. Statistical analysis was done using JMP and means compared using Student’s t-test (Significance at P < 0.05). In study 1, EBMet samples were incubated in the rumen of 2 cannulated cows for 16 h. Rumen undegradable N (RUN, % of N) content of EBMet was determined to be 91.8% (SEM = 0.38). In study 2, using 2 steers fitted with rumen and duodenal cannulas, the RUP, intestinal digestibility of RUP (dRUP), and total-tract CP digestibility (TTCPDig %) of EBMet were determined with the mobile bag technique and Soypass as a reference. RUP was not different between EBMet and CON (91.3 vs. 102.7, SEM = 2.79) nor was dRUP (98.9 vs. 100.0, SEM = 0.36) or TTCPDig (99.0 vs 100.0, SEM = 0.30). Study 3, a completely randomized design, measured plasma AA in 8 lactating Holstein cows (DIM 126 ± 19, MY 42.4 kg/d, Parity = 2). Four cows were fed a diet with no Met supplementation (0Met) and 4 cows fed the same diet with 170 g/d of EBMet. Diets were fed for 5 d and blood from the coccygeal vein was collected at 1000, 1200, 1400, and 1600 h on d 5. Plasma Met (μg/mL) was 3.29 in EBMet fed cows and higher (P < 0.05) than the 2.78 of 0Met fed cows (SEM = 0.15). Met as % of Total AA (1.10 vs. 0.91, SEM = 0.03) and % of Essential AA (2.29 vs. 1.91, SEM = 0.08) was higher (P < 0.05) for EBMet than 0Met fed cows. In study 4 on a commercial farm in Minnesota, ­8 Holstein cows (DIM 117 ± 56, MY 39.6 kg/d, Parity = 2.1) were fed a CON diet (no Met supplementation) for 7 d followed by 7 d of CON with EBMet (170 g/d). Blood from the coccygeal vein was collected on d 7 of each feeding period at 1000 and 1400 h. Cows fed EBMet had higher (P < 0.05) concentration of plasma Met (μg/mL) (3.27 vs. 2.81, SEM = 0.12), Met as % of Total AA (1.37 vs. 1.03, SEM = 0.04) and Met as % of Essential AA (3.25 vs. 2.35, SEM = 0.10) than CON fed cows. Data suggests EBMet is a highly metabolizable Met source as shown by RUP, dRUP, TTCPDig exceeding 90.0% and increased plasma Met concentrations in vivo.

Key Words: methionine, amino acid, blood plasma