The objectives were to investigate if increasing supply of methionine during late-pregnancy in Holstein cows affects offspring plasma amino acid (AA) concentration and hepatic one-carbon (1C) metabolism. Calves were born to cows fed a control diet (CON) or the control plus rumen-protected methionine (MET) for the last 28 d prepartum (0.09% of DMI), and were fed and managed similarly after birth. Plasma samples and liver biopsies were harvested at 4, 14, 28, and 50 d of age and used for AA, mRNA expression and activity of hepatic 1C metabolism enzymes. Data were analyzed using a Mixed model considering block as random effect and treatment, time and its interaction as fixed effect. At birth, only the concentrations of plasma His and Met differed due to maternal treatment because of lower concentrations in MET calves. After birth, the overall concentrations of Met, Glu, and Leu was greater in MET compared with CON calves. There was an interaction of treatment× day (T×D) for the concentration of Tyr, Phe, Gln, Arg, Thr and Tau due to greater levels early in life in MET compared with CON calves. A T×D was observed for the activity of betaine-homocysteine S-methyltransferase (BHMT), methionine synthase enzyme (MTR), and cystathionine-β-synthase (CBS) in liver; MET calves had greater BHMT activity on d 14 and greater CBS on d 4 and peaked at 28. In contrast, despite a linear increase from d 4 to 28, activity of MTR in MET calves was lower on d 4 and 50. Methionine adenosyltransferase 1A (MAT1A) mRNA expression was greater overall in MET compared with CON calves. Greater mRNA expression of betaine-homocysteine S-methyltransferase 2 (BHMT2) and CBS in MET calves was observed on d 4 and 14. Along with CBS, the greater expression of cysteine dioxygenase 1 (CDO1) as well as glutathione reductase (GSR) and glutathione peroxidase (GPX1) on d 4, 14, and 28 in MET calves suggested greater synthesis of glutathione and taurine. Overall, the data indicate that enhanced methionine supply during the last 30 d of gestation not only can benefit calf performance, but increase AA availability and activity of the 1C metabolism pathway to furnish cells with antioxidants.